Detects endogenous human, mouse, and rat CK1 gamma in Western blots. In Western blots, the immunogen is derived from a region of human CK1 gamma that is 97% to 98% identical to human CK1 gamma 2 and CK1 gamma 3, so all three isoforms should be detected.
Polyclonal Sheep IgG
E. coli-derived recombinant human CK1 gamma Val182-Tyr304 Accession # Q9HCP0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat Casein Kinase 1 gamma by Western Blot. Western blot shows lysates of human, mouse and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Casein Kinase 1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3186) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CK1 gamma isoforms at approximately 45 to 51 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Casein Kinase 1 gamma in Human Brain. Casein Kinase 1 gamma was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Sheep Anti-Human/Mouse/Rat Casein Kinase 1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3186) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Casein Kinase 1 gamma
Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, beta, gamma 1, gamma 2, gamma 3, δ and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. The C-termini of the gamma isoforms carry putative palmitoylation sites unique within the CK1 family that are believed to anchor the proteins in the plasma membrane.
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