Human/Mouse/Rat Cubilin Antibody

Catalog # Availability Size / Price Qty
AF3700
AF3700-SP
Human/Mouse/Rat Cubilin Antibody in Data
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Product Details
Citations (2)
FAQs
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Human/Mouse/Rat Cubilin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Cubilin in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Cubilin
Ser36-Gly126
Accession # O60494
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Cubilin antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Cubilin by Western Blot. Western blot shows lysates of human, mouse, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/ Rat Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody HAF016). A specific band was detected for Cubilin at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cubilin antibody in Human Kidney Tissue by Immunohistochemistry (IHC-P). View Larger

Cubilin in Human Kidney Tissue. Cubilin was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Sheep Anti-Human/Mouse/Rat Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (brown; (VC006) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Mouse Cubilin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Cubilin by Simple WesternTM. Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Cubilin at approximately 500 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cubilin

Cubilin is a 460 kDa protein that is a component of a multiligand endocytic receptor complex, which also contains amnionless, megalin and LRP2. The receptor complex is expressed in several absorptive epithelia, including kidney proximal tubule, yolk sac, and the small intestine. Human Cubilin is synthesized as a 3623 residue precursor with a signal peptide, an amino-terminal domain, eight EGF-like repeats and 27 Cub domains. Co-expression with amnionless is required for the membrane anchoring, ER export and internalizing action of Cubilin. Within the region used as the immunogen, human, mouse and rat Cubilin share approximately 76% amino acid sequence homology.

Entrez Gene IDs
8029 (Human); 65969 (Mouse); 80848 (Rat)
Alternate Names
cubilin; cubilin (intrinsic factor-cobalamin receptor); Cubilin; CUBN; Cubulin; gp280; IFCR; MGA1

Product Datasheets

Citations for Human/Mouse/Rat Cubilin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Genetic Analyses in Dent Disease and Characterization of CLCN5 Mutations in Kidney Biopsies
    Authors: L Gianesello, M Ceol, L Bertoldi, L Terrin, G Priante, L Murer, L Peruzzi, M Giordano, F Paglialong, V Cantaluppi, C Musetti, G Valle, DD Prete, F Anglani, DDI Network
    Int J Mol Sci, 2020;21(2):.
    Species: Human
    Sample Types: Tissue
    Applications: IF
  2. Characterizing interactions of Leptospira interrogans with proximal renal tubule epithelial cells
    Authors: T Yamaguchi, N Higa, N Okura, A Matsumoto, I Hermawan, T Yamashiro, T Suzuki, C Toma
    BMC Microbiol., 2018;18(1):64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Secondary Antibodies

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