Cubilin Antibody

R&D Systems | Catalog # AF3700

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Cubilin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cubilin
Ser36-Gly126
Accession # O60494

Specificity

Detects human Cubilin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Cubilin Antibody

Detection of Human, Mouse, and Rat Cubilin antibody by Western Blot.

Detection of Human, Mouse, and Rat Cubilin by Western Blot.

Western blot shows lysates of human, mouse, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/ Rat Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody HAF016). A specific band was detected for Cubilin at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cubilin antibody in Human Kidney Tissue by Immunohistochemistry (IHC-P).

Cubilin in Human Kidney Tissue.

Cubilin was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Sheep Anti-Human/Mouse/Rat Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (brown; (VC006) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse Cubilin antibody by Simple WesternTM.

Detection of Mouse Cubilin by Simple WesternTM.

Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Cubilin at approximately 500 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Cubilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3700) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Detection of Cubilin by Immunohistochemistry

Detection of Cubilin by Immunohistochemistry

Immunofluorescence staining of Cubilin in developing control (ctrl) and yotari (yot) lung tissue (a–d). Comparative expression of Cubilin in the lungs at embryonic day 13.5 (E13.5) and 15.5 (E15.5) is shown in (a–d). Arrows in each substructure of the lungs indicate positive staining of Cubilin. Magnification: ×40; scale bar: 100 µm. The area percentage of Cubilin in control specimens (ctrl) and yotari mice per structure (epithelium and mesenchyme) throughout different stages of developing lungs (e,f). Two substructures were assessed at E13.5 and E15.5. Data are displayed as the mean ± SD (vertical line) and analyzed by a two-way ANOVA test followed by Tukey’s multiple comparison test. The statistical details supporting these findings are provided in Supplementary Tables S1 and S2. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2075-1729/15/9/1395), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Cubilin by Immunohistochemistry

Detection of Cubilin by Immunohistochemistry

Double immunofluorescence staining of embryonic wild-type and yotari mouse kidneys was performed using Megalin (a–d) and Cubilin (a–d) markers. Arrows indicate the immunoexpression patterns of Megalin and Cubilin in the metanephric mesenchyme (mm), glomeruli (g), renal vesicles (rv), ampullae (A), convoluted tubules (Ct) and collecting ducts (Cd), as shown on the 4′,6-diamidino-2-phenylindole (DAPI) nuclei staining image. Immunoexpression of Megalin, Cubilin, DAPI staining and the merged images of Megalin, Cubilin and DAPI was observed at embryonic days E13.5 and E15.5 in wild-type (a,c) and yotari (b,d) kidneys. An asterisk marks the region where co-expression was detected. Images were taken at ×40 magnification, and the scale bar represents 100 μm for all images. The percentages of Megalin (e) and Cubilin (f) positive cells in the metanephric mesenchyme (mm), renal vesicles (rv) or glomeruli (g), convoluted tubules (Ct) and ampullae (A) or collecting ducts (Cd) of wild-type and yotari kidneys at embryonic days 13.5 and 15.5 are shown. Data are presented as the mean ± SD (vertical line) and analyzed using a two-way ANOVA followed by Tukey’s multiple comparison test. Significant differences are indicated by * p < 0.05 and **** p < 0.0001. Ten substructures were assessed at each time point. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2227-9059/12/7/1542), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Cubilin by Immunohistochemistry

Detection of Cubilin by Immunohistochemistry

Double immunofluorescence staining of postnatal wild-type and yotary mouse kidneys was performed using Megalin (a,b) and Cubilin (a,b) markers. Arrows show the immunoexpression patterns of Megalin and Cubilin in glomeruli (g), distal convoluted tubules (dct) and proximal convoluted tubules (pct), indicated on 4′,6-diamidino-2-phenylindole (DAPI) nuclei staining image. Immunoexpression of Megalin and Cubilin staining and merged Megalin, Cubilin and DAPI at 4 days (P4) and 14 days (P14) in wild type. Wild-type and yotari mice at postnatal days 4 and 14 of kidney development predominantly matched the localization and intensity of Megalin and Cubilin. Therefore, representative images were taken from wild-type mice. An asterisk marks the region where co-expression was detected. Images were taken at ×40 magnification, and the scale bar represents 100 μm for all images. The percentages of Megalin (c) and Cubilin (d) positive cells in the glomeruli (G), distal convoluted tubules (DCT) and proximal convoluted tubules (PCT) of postnatal kidneys of wild type and yotari animals over time (P4 and P14). Data are presented as the mean ± SD (vertical line) and analyzed using a two-way ANOVA followed by Tukey’s multiple comparison test. Significant differences are indicated by **** p < 0.0001. Twenty substructures were assessed at each time point. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2227-9059/12/7/1542), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Cubilin Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney tissue

Simple Western

10 µg/mL
Sample: Mouse kidney tissue

Western Blot

1 µg/mL
Sample: Human, mouse, and rat kidney tissue

Reviewed Applications

Read 2 reviews rated 4.5 using AF3700 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cubilin

Cubilin is a 460 kDa protein that is a component of a multiligand endocytic receptor complex, which also contains amnionless, megalin and LRP2. The receptor complex is expressed in several absorptive epithelia, including kidney proximal tubule, yolk sac, and the small intestine. Human Cubilin is synthesized as a 3623 residue precursor with a signal peptide, an amino-terminal domain, eight EGF-like repeats and 27 Cub domains. Co-expression with amnionless is required for the membrane anchoring, ER export and internalizing action of Cubilin. Within the region used as the immunogen, human, mouse and rat Cubilin share approximately 76% amino acid sequence homology.

Alternate Names

CUBN, Cubulin, gp280, IFCR, MGA1

Entrez Gene IDs

8029 (Human); 65969 (Mouse); 80848 (Rat)

Gene Symbol

CUBN

UniProt

O60494

Additional Cubilin Products

Product Documents for Cubilin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Cubilin Antibody

For research use only

Citations for Cubilin Antibody

Customer Reviews for Cubilin Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Great antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: HK-2
    Species: Human
    Verified Customer | Posted 07/28/2025
    HK2 cells cultured on glass coverslips stained with Cubilin antibody and DAPI.
    Antigen retrieval Citrate buffer on pressure cooker. Block 1h RT 5% Donkey serum. Primary ab 1:200 4ºC overnight in humidified chamber. Secondary ab Dk anti Goat 647 2h RT. Antibodies diluted in 1% Dk serum.
    Cubilin Antibody AF3700
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Kidney tissue homogenates
    Species: Mouse
    Verified Customer | Posted 12/19/2014

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