Detects DGK-theta in direct ELISAs and Western blots. In direct ELISAs and Western blolts, no cross-reactivity with recombinant human DGK-alpha, -beta, -gamma, -epsilon, -iota, -kappa, or -zeta is observed.
Monoclonal Mouse IgG1 Clone # 652308
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human DGK-theta Asp696-Lys839 Accession # P52824
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat DGK-theta by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, TF‑1 human erythroleukemic cell line, HEK293 human embryonic kidney cell line, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Human DGK-theta Monoclonal Antibody (Catalog # MAB6598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-theta at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Diacylglycerol kinase theta (DGK-theta ) is an approximately 110 kDa kinase that phosphorylates the lipid
diacylglycerol. DGK-theta binds to RhoA, Akt, PKC epsilon and eta, and
Steroidogenic Factor. It translocates to the nucleus or to the plasma membrane
in response to NGF, TPA, or GPCR stimulation (thrombin, bradykinin,
norepinephrine). DGK-theta contains three Cys-rich zinc finger regions (aa
60-108, aa 121-168, and aa 183-234), a Ras-associating domain (aa 395-494), two
LxxLL motifs (aa 555-559 and aa 574-578), and a catalytic domain (aa 590-714). Over
aa 696-839, human DGK-theta shares 90% aa sequence identity with mouse and rat DGK-theta.
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