Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 652308
View all DGK-theta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DGK-theta
Asp696-Lys839
Accession # P52824

Specificity

Detects DGK-theta in direct ELISAs and Western blots. In direct ELISAs and Western blolts, no cross-reactivity with recombinant human DGK-alpha, -beta, -gamma, -epsilon, -iota, -kappa, or -zeta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat DGK-theta Antibody

Detection of Human, Mouse, and Rat DGK-? antibody by Western Blot.

Detection of Human, Mouse, and Rat DGK-theta by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, TF-1 human erythroleukemic cell line, HEK293 human embryonic kidney cell line, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Human DGK-theta Monoclonal Antibody (Catalog # MAB6598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-theta at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat DGK-theta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, TF‑1 human erythroleukemic cell line, HEK293 human embryonic kidney cell line, mouse brain tissue, and rat brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DGK-theta

Diacylglycerol kinase theta (DGK-theta ) is an approximately 110 kDa kinase that phosphorylates the lipid diacylglycerol. DGK-theta binds to RhoA, Akt, PKC epsilon and eta, and Steroidogenic Factor. It translocates to the nucleus or to the plasma membrane in response to NGF, TPA, or GPCR stimulation (thrombin, bradykinin, norepinephrine). DGK-theta contains three Cys-rich zinc finger regions (aa 60-108, aa 121-168, and aa 183-234), a Ras-associating domain (aa 395-494), two LxxLL motifs (aa 555-559 and aa 574-578), and a catalytic domain (aa 590-714). Over aa 696-839, human DGK-theta shares 90% aa sequence identity with mouse and rat DGK-theta.

Long Name

Diacylglycerol Kinase, theta 110kDa

Alternate Names

DGKQ, DGKtheta

Entrez Gene IDs

1609 (Human)

Gene Symbol

DGKQ

UniProt

P52824

Additional DGK-theta Products

Product Documents for Human/Mouse/Rat DGK-theta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat DGK-theta Antibody

For research use only

Related Research Areas

Intracellular Kinases

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