Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 563518
View all DYRK3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DYRK3
Asn52-Glu167
Accession # O43781

Specificity

Detects human, mouse, and rat DYRK3 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat DYRK3 Antibody

Detection of Human, Mouse, and Rat DYRK3 antibody by Western Blot.

Detection of Human, Mouse, and Rat DYRK3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat DYRK3 Monoclonal Antibody (Catalog # MAB5409) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for DYRK3 at approximately 70 kDa and 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat DYRK3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DYRK3

DYRK3 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 3; also REDK) is a 70‑72 kDa member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. It is expressed in testis and erythroid-lineage precursors, and shows dual substrate specificity; autophosphorylation on Tyr369 to self‑activate, and a Ser/Thr phosphorylation of target molecules. Substrates include CREB and Histone 2B. Human DYRK3 is 588 amino acids (aa) in length and contains one kinase catalytic domain (aa 209‑522). There are two potential isoform variants that show either a six aa substitution for aa 1‑26, or this same substitution in combination with a premature truncation after Gln263. DYRK3 immunoreactive proteolysis bands of 30 and 40 kDa have been detected in DYRK3-transfected cells. Over aa 52‑167, human DYRK3 shares 75% aa identity with mouse DYRK3.

Long Name

Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 3

Alternate Names

DYRK5, REDK, YAK3-2

Entrez Gene IDs

8444 (Human); 226419 (Mouse); 304775 (Rat)

Gene Symbol

DYRK3

UniProt

O43781

Additional DYRK3 Products

Product Documents for Human/Mouse/Rat DYRK3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat DYRK3 Antibody

For research use only

Related Research Areas

Intracellular Kinases

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