DYRK3 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 3; also REDK) is a 70‑72 kDa member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. It is expressed in testis and erythroid-lineage precursors, and shows dual substrate specificity; autophosphorylation on Tyr369 to self‑activate, and a Ser/Thr phosphorylation of target molecules. Substrates include CREB and Histone 2B. Human DYRK3 is 588 amino acids (aa) in length and contains one kinase catalytic domain (aa 209‑522). There are two potential isoform variants that show either a six aa substitution for aa 1‑26, or this same substitution in combination with a premature truncation after Gln263. DYRK3 immunoreactive proteolysis bands of 30 and 40 kDa have been detected in DYRK3-transfected cells. Over aa 52‑167, human DYRK3 shares 75% aa identity with mouse DYRK3.
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Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Asn52-Glu167
Accession # O43781
Specificity
Clonality
Host
Isotype
Scientific Data Images for DYRK3 Antibody (563518)
Detection of Human, Mouse, and Rat DYRK3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat DYRK3 Monoclonal Antibody (Catalog # MAB5409) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for DYRK3 at approximately 70 kDa and 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for DYRK3 Antibody (563518)
Western Blot
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: DYRK3
Long Name
Alternate Names
Gene Symbol
UniProt
Additional DYRK3 Products
Product Documents for DYRK3 Antibody (563518)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for DYRK3 Antibody (563518)
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars