Human/Mouse/Rat EEA1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat EEA1 by Western Blot.
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Product Details
Citations (4)
Supplemental Products

Human/Mouse/Rat EEA1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat EEA1 in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human EEA1
Accession # Q15075
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat EEA1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat EEA1 by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and Sheep Anti-Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat EEA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8047) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EEA1 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry EEA1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

EEA1 in HeLa Human Cell Line. EEA1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat EEA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8047) at 1.7 and 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to early endosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EEA1

EEA1 (Early Endosome Autoantigen 1; also Endosome-associated protein p162 and Zn-finger FYVE domain-containing protein 2) is a 170-180 kDa protein that serves as an identifying marker for early endosomes. It is ubiquitously expressed, and found in both the cytosol and on cellular membranes. Its activity has been described as that of a tethering factor which links endosomes to endocytic vesicles, allowing for their fusion via a SNARE complex. Normally, EEA1 exists as a homodimer in the cytoplasm and appears to make transient contacts with endosome membrane phosphatidylinositol. When endosome fusion is not required, EEA1 serves as a substrate for p97, promoting EEA1 dissociation and endosome independence. When endosome fusion is required, EEA1 interacts with NSF, resulting in its removal from a large endosome-associated complex and subsequent endosomal vesicle fusion. Human EEA1 is synthesized as a 1411 amino acid (aa) protein that contains one C2H2-type Zn finger region (aa 41-64) and one FYVE Zn finger domain (aa 1352-1410). There is one isoform variant that contains a nine aa substitution for aa 925-1411. Over aa 1249-1356, human EEA1 shares 99% aa sequence identity with mouse EEA1.

Long Name
Early Endosome Antigen 1
Entrez Gene IDs
8411 (Human); 216238 (Mouse); 314764 (Rat)
Alternate Names
early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; EEA1; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; ZFYVE2MST105; Zinc finger FYVE domain-containing protein 2

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Citations for Human/Mouse/Rat EEA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. SIDT1 plays a key role in type I IFN responses to nucleic acids in plasmacytoid dendritic cells and mediates the pathogenesis of an imiquimod-induced psoriasis model
    Authors: M Morell, N Varela, C Castillejo, C Coppard, MJ Luque, YY Wu, N Martín-Mor, F Pérez-Cóza, G Gómez-Hern, R Kumar, F O'Valle, ME Alarcón-Ri, C Marañón
    EBioMedicine, 2022-01-19;76(0):103808.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis
    Authors: Y Zhu, Z Wu, W Yan, F Shao, B Ke, X Jiang, J Gao, W Guo, Y Lai, H Ma, D Chen, Q Xu, Y Sun
    Embo Molecular Medicine, 2021-12-22;0(0):e14455.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Spatial and temporal VEGF receptor intracellular trafficking in microvascular and macrovascular endothelial cells
    Authors: JAF Silva, X Qi, MB Grant, ME Boulton
    Scientific Reports, 2021-08-30;11(1):17400.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  4. EGFL7 enhances surface expression of integrin ?5?1 to promote angiogenesis in malignant brain tumors
    Authors: N Dudvarski, F Bicker, S Keller, DT Jones, PN Harter, A Kienzle, C Gillmann, P Arnold, A Golebiewsk, O Keunen, A Giese, A von Deimli, T Bäuerle, SP Niclou, M Mittelbron, W Ye, SM Pfister, MH Schmidt
    EMBO Mol Med, 2018-09-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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