Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody

Catalog # Availability Size / Price Qty
AF5169
AF5169-SP
Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody in Data
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Product Details
Citations (1)
FAQs
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Reviews (4)

Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human and mouse Enolase 2/Neuron-specific Enolase in direct ELISAs. Detects human, mouse, and rat Enolase 2/Neuron-specific Enolase in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Enolase 2/Neuron-specific Enolase
Met1-Leu434
Accession # P09104
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2-1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse Enolase 2 by Western Blot. View Larger

Detection of Human/Mouse Enolase 2 by Western Blot. Western blot shows lysates of human brain, mouse brain tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Enolase 2 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Western Blot Detection of Rat Enolase 2/Neuron‑specific Enolase by Western Blot. View Larger

Detection of Rat Enolase 2/Neuron‑specific Enolase by Western Blot. Western blot shows lysates of rat brain tissue, rat cerebellum tissue, and rat olfactroy bulb tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Enolase 2/Neuron-specific Enolase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Enolase 2/Neuron-specific Enolase at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Enolase 2 in Human Brain. View Larger

Enolase 2 in Human Brain. Enolase 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Enolase 2 in Human Brain. View Larger

Enolase 2 in Human Brain. Enolase 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Enolase 2/Neuron‑specific Enolase by Simple Western<sup>TM</sup>. View Larger

Detection of Human Enolase 2/Neuron‑specific Enolase by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for Enolase 2/Neuron-specific Enolase at approximately 50 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse Enolase 2/Neuron-specific Enolase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Enolase 2/Neuron-specific Enolase

Enolase 2 (2-phospho-D-glycerate hydrolyase; also neural enolase and gamma -enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha -enolase. Full-length human Enolase 2 is 99% aa identical to both mouse and canine Enolase 2. It shares 83% aa identity with human enolases # 1 and # 3.

Entrez Gene IDs
2026 (Human); 13807 (Mouse); 24334 (Rat)
Alternate Names
2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; EC 4.2.1.11; ENO2; enolase 2 (gamma, neuronal); Enolase 2; gamma-Enolase; Neural enolase; neuron specific gamma enolase; neurone-specific enolase; Neuronspecific Enolase; Neuron-specific enolase; NSE

Product Datasheets

Citation for Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inflammation-induced reversible switch of the neuron-specific enolase promoter from Purkinje neurons to Bergmann glia
    Authors: Yusuke Sawada
    Sci Rep, 2016;6(0):27758.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody

Average Rating: 4 (Based on 4 Reviews)

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Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human