Enolase 2/Neuron-specific Enolase Antibody

R&D Systems | Catalog # AF5169

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Enolase 2/Neuron-specific Enolase Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Enolase 2/Neuron-specific Enolase
Met1-Leu434
Accession # P09104

Specificity

Detects human and mouse Enolase 2/Neuron-specific Enolase in direct ELISAs. Detects human, mouse, and rat Enolase 2/Neuron-specific Enolase in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody

Detection of Human/Mouse Enolase 2 antibody by Western Blot.

Detection of Human/Mouse Enolase 2 by Western Blot.

Western blot shows lysates of human brain, mouse brain tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Enolase 2 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Rat Enolase 2/Neuron-specific Enolase antibody by Western Blot.

Detection of Rat Enolase 2/Neuron‑specific Enolase by Western Blot.

Western blot shows lysates of rat brain tissue, rat cerebellum tissue, and rat olfactroy bulb tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Enolase 2/Neuron-specific Enolase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Enolase 2/Neuron-specific Enolase at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Enolase 2 antibody in Human Brain by Immunohistochemistry (IHC-P).

Enolase 2 in Human Brain.

Enolase 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Enolase 2 antibody in Human Brain by Immunohistochemistry (IHC-P).

Enolase 2 in Human Brain.

Enolase 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Enolase 2/Neuron-specific Enolase antibody by Simple WesternTM.

Detection of Human Enolase 2/Neuron‑specific Enolase by Simple WesternTM.

Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for Enolase 2/Neuron-specific Enolase at approximately 50 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse Enolase 2/Neuron-specific Enolase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Enolase 2/Neuron-specific Enolase Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Simple Western

10 µg/mL
Sample: BG01V human embryonic stem cells

Western Blot

0.2-1 µg/mL
Sample: Human brain, mouse brain tissue, BG01V human embryonic stem cells, rat brain tissue, rat cerebellum tissue, and rat olfactroy bulb tissue

Reviewed Applications

Read 4 reviews rated 4 using AF5169 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Enolase 2/Neuron-specific Enolase

Enolase 2 (2-phospho-D-glycerate hydrolyase; also neural enolase and gamma -enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha -enolase. Full-length human Enolase 2 is 99% aa identical to both mouse and canine Enolase 2. It shares 83% aa identity with human enolases # 1 and # 3.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Entrez Gene IDs

2026 (Human); 13807 (Mouse); 24334 (Rat)

Gene Symbol

ENO2

UniProt

P09104

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Neuronal Markers

Citations for Enolase 2/Neuron-specific Enolase Antibody

Customer Reviews for Enolase 2/Neuron-specific Enolase Antibody (4)

4 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
50%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    Enolase 2/Neuron-specific Enolase Antibody AF5169
  • Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018
  • Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human/Mouse/Rat Enolase 2/Neuron-specific Enolase Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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Protocols

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