Human/Mouse/Rat Ets-1 Antibody

Catalog # Availability Size / Price Qty
AF7284
AF7284-SP
Detection of Human, Mouse, and Rat Ets-1 by Western Blot.
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Human/Mouse/Rat Ets-1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Ets-1 in Western blots and detects recombinant human Ets-1 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Ets-1
Glu127-Val230
Accession # P14921
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Ets-1 by Western Blot. View Larger

Detection of Human, Mouse, and Rat Ets-1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, human thymus tissue, mouse thymus tissue, and rat CD4+ T cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Ets-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7284) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ets-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Ets‑1  in Human Ovary. View Larger

Ets‑1 in Human Ovary. Ets‑1 was detected in immersion fixed paraffin-embedded sections of human ovary using Sheep Anti-Human/Mouse/Rat Ets‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7284) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ets-1

Ets-1 (E26 Transformation-Specific 1; also p54 and c-ets1) is a 52-54 kDa member of the ETS family of proteins. It is found in multiple cell types, and serves as a transcriptional regulator (generally activator) of multiple target genes, including prolactin, the transferrin receptor, and Cyclin E. By upregulating Cyclin E and CDK2 genes, it promotes cell-cycle progression. Ets‑1 forms complexes with both transcriptional activators (AP-1 and GHF-1) and repressors (MafB and Daxx). Human Ets‑1 is 441 amino acids (aa) in length. It contains one PNT domain (aa 51-136) that binds ERK2, and a DNA-binding ETS domain (aa 335-415). There are two SUMOylation sites, plus four utilized phosphorylation and acetylated lysine sites. At least four potential isoform variants are reported. One shows a deletion of aa 244-330 (termed isoform 1B), a second shows a deletion of aa 262-331, a third contains a deletion of aa 28-244, and a fourth possesses an 11 aa substitution for aa 262-441. Over aa 127-230, human Ets‑1 shares 95% aa identity with mouse Ets‑1.

Long Name
E26 Transformation-specific Sequence-1
Entrez Gene IDs
2113 (Human); 23871 (Mouse)
Alternate Names
Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; ets protein; Ets1; Ets-1; EWSR2; FLJ10768; p54; Protein C-Ets-1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); v-ets

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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