Human/Mouse/Rat Ets-1 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Ets-1 in Western blots and detects recombinant human Ets-1 in direct ELISAs.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Ets-1
    Glu127-Val230
    Accession # P14921
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Ets-1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, human thymus tissue, mouse thymus tissue, and rat CD4+ T cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Ets-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7284) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ets-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ets-1

Ets-1 (E26 Transformation-Specific 1; also p54 and c-ets1) is a 52-54 kDa member of the ETS family of proteins. It is found in multiple cell types, and serves as a transcriptional regulator (generally activator) of multiple target genes, including prolactin, the transferrin receptor, and Cyclin E. By upregulating Cyclin E and CDK2 genes, it promotes cell-cycle progression. Ets‑1 forms complexes with both transcriptional activators (AP-1 and GHF-1) and repressors (MafB and Daxx). Human Ets‑1 is 441 amino acids (aa) in length. It contains one PNT domain (aa 51-136) that binds ERK2, and a DNA-binding ETS domain (aa 335-415). There are two SUMOylation sites, plus four utilized phosphorylation and acetylated lysine sites. At least four potential isoform variants are reported. One shows a deletion of aa 244-330 (termed isoform 1B), a second shows a deletion of aa 262-331, a third contains a deletion of aa 28-244, and a fourth possesses an 11 aa substitution for aa 262-441. Over aa 127-230, human Ets‑1 shares 95% aa identity with mouse Ets‑1.

  • Long Name:
    E26 Transformation-specific Sequence-1
  • Entrez Gene IDs:
    2113 (Human); 23871 (Mouse)
  • Alternate Names:
    Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; ets protein; Ets1; Ets-1; EWSR2; FLJ10768; p54; Protein C-Ets-1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); v-ets
Related Research Areas
Isotype Controls
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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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