Human/Mouse/Rat FANCD2 Antibody

MAB93691 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB93691-100
MAB93691-SP

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Detection of Human, Mouse, and Rat FANCD2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FANCD2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human FANCD2 in direct ELISAs. Detects human, mouse, and rat FANCD2 in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1290C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E.coli-derived recombinant human FANCD2
Glu11-Glu230
Accession # Q9BXW9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Knockout Validated

FANCD2 is specifically detected in HeLa humancervical epithelial carcinoma parental cell line but is not detectable inFANCD2 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Human, Mouse, and Rat FANCD2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat FANCD2 Monoclonal Antibody (Catalog # MAB93691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human FANCD2 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human FANCD2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat FANCD2 Monoclonal Antibody (Catalog # MAB93691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FANCD2

Fanconi anemia group D2 protein, encoded by the FANCD2 gene, is a member of the Fanconi anemia complementation (FANC) group that also includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCE, FANCF, FANCG, and FANCL. The members of the Fanconi anemia complementation group share little sequence similarities.

Long Name

Fanconi Anemia, Complementation Group D2

Entrez Gene IDs

2177 (Human); 211651 (Mouse); 312641 (Rat)

Alternate Names

DKFZp762A223; FA4; FACD; FAD; FAD2; FA-D2; FADFAD2; FANCD; FANCD2; Fanconi anemia complementation group D2; Fanconi anemia group D2 protein; Fanconi anemia, complementation group D2; FLJ23826; FPN1; HFE4; IREG1; Protein FACD2; SLC11A3