FANCD2 Antibody (1290C)

Detection of FANCD2 by Western Blot

CF10 activates the ATR/Chk1 pathway, consistent with replication stress, and causes DNA DSBs in CC531 cells. Western blots from CC531 cell lysates: (A) CF10 dose–response graphs demonstrate that a 250 nM dose is sufficient for ATR/Chk1 pathway activation and DNA DSBs; (B) equimolar 5-FU is not effective in ATR/Chk1 activation and DNA DSBs; and (C) a 10-fold greater 5-FU dose is relatively less effective than 250 nM CF10. * indicates mono-ubiquitnated FANCD2. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2072-6694/16/7/1360), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FANCD2 by Western Blot

CF10 activates the ATR/Chk1 pathway, consistent with replication stress, and causes DNA DSBs in CC531 cells. Western blots from CC531 cell lysates: (A) CF10 dose–response graphs demonstrate that a 250 nM dose is sufficient for ATR/Chk1 pathway activation and DNA DSBs; (B) equimolar 5-FU is not effective in ATR/Chk1 activation and DNA DSBs; and (C) a 10-fold greater 5-FU dose is relatively less effective than 250 nM CF10. * indicates mono-ubiquitnated FANCD2. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2072-6694/16/7/1360), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of afpdau30tbxqqdhcvrla.jpg by Western Blot

LIN37 suppresses HR protein expression in non-cycling cells.(A) Gene ontology (GO) analysis of genes upregulated in non-cycling Lig4−/−:Lin37−/− abl pre-B cells. Enriched GO terms with the 20 lowest p-values (on the right of each bar) are shown. (B) Western blot analysis of indicated proteins in cycling or non-cycling WT, Trp53bp1−/−, and Lin37−/− abl pre-B cells. HR, homologous recombination; WT, wild type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34477552), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of beax31b3yqaeebr9muas.jpg by Western Blot

LIN37 function in DNA end protection is restricted to G0.(A, B) Western blot analysis of indicated proteins in cycling and non-cycling abl pre-B cells (A) or MCF10A cells (B). (C, D) Western blot analysis of indicated proteins in cycling G1 or S/G2/M abl pre-B cells (C) or MCF10A cells (D), isolated by flow cytometric cell sorting based on the PIP-FUCCI reporter. Representative of two independent experiments. Asterisk indicates non-specific recognizing bands. (E, F) Flow cytometric analysis of chromatin-bound RPA and gamma H2AX before and after IR treatment of G1-phase Lig4−/−, Lig4−/−:Trp53bp1−/− and Lig4−/−:Lin37−/− abl pre-B cells (E) or WT, Trp53bp1−/−, and Lin37−/− MCF10A cells (F). Representative of three experiments. IR, ionizing radiation; WT, wild type.Figure 7—source data 1.RNA-Seq result and GO analysis in cycling G1Lig4−/− and Lig4−/−:Lin37−/− abl pre-B cells.GO, gene ontology; RNA-Seq, RNA sequencing.Figure 7—source data 2.GO analysis of genes upregulated in non-cycling G0 and/or cycling G1Lig4−/−:Lin37−/− abl pre-B cells.GO, gene ontology.RNA-Seq result and GO analysis in cycling G1Lig4−/− and Lig4−/−:Lin37−/− abl pre-B cells.GO, gene ontology; RNA-Seq, RNA sequencing.GO analysis of genes upregulated in non-cycling G0 and/or cycling G1Lig4−/−:Lin37−/− abl pre-B cells.GO, gene ontology.Identification of G1-phase cells in proliferating cells.(A, B) Flow cytometric analysis of EdU incorporation and DNA content (7-AAD) of cycling Lig4−/−, Lig4−/−:Trp53bp1−/−, and Lig4−/−:Lin37−/− abl pre-B cells (A) or cycling WT, Trp53bp1−/−, and Lin37−/− MCF10A cells (B). Cells were treated (+EdU) or not treated (−EdU) with EdU and analyses were carried out before or after IR. The percentage of G1-phase cells is shown. IR, ionizing radiation; WT, wild type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34477552), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of bsj8zokabieoi4xhbttv.jpg by Western Blot

CF10 activates the ATR/Chk1 pathway, consistent with replication stress, and causes DNA DSBs in CC531 cells. Western blots from CC531 cell lysates: (A) CF10 dose–response graphs demonstrate that a 250 nM dose is sufficient for ATR/Chk1 pathway activation and DNA DSBs; (B) equimolar 5-FU is not effective in ATR/Chk1 activation and DNA DSBs; and (C) a 10-fold greater 5-FU dose is relatively less effective than 250 nM CF10. * indicates mono-ubiquitnated FANCD2. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2072-6694/16/7/1360), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of c7qkvvj0koq9cvooruff.jpg by Western Blot

CF10 activates the ATR/Chk1 pathway, consistent with replication stress, and causes DNA DSBs in CC531 cells. Western blots from CC531 cell lysates: (A) CF10 dose–response graphs demonstrate that a 250 nM dose is sufficient for ATR/Chk1 pathway activation and DNA DSBs; (B) equimolar 5-FU is not effective in ATR/Chk1 activation and DNA DSBs; and (C) a 10-fold greater 5-FU dose is relatively less effective than 250 nM CF10. * indicates mono-ubiquitnated FANCD2. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2072-6694/16/7/1360), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of gx3yg3gne4afqelpe86c.jpg by Western Blot

CF10 activates the ATR/Chk1 pathway, consistent with replication stress, and causes DNA DSBs in CC531 cells. Western blots from CC531 cell lysates: (A) CF10 dose–response graphs demonstrate that a 250 nM dose is sufficient for ATR/Chk1 pathway activation and DNA DSBs; (B) equimolar 5-FU is not effective in ATR/Chk1 activation and DNA DSBs; and (C) a 10-fold greater 5-FU dose is relatively less effective than 250 nM CF10. * indicates mono-ubiquitnated FANCD2. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2072-6694/16/7/1360), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FANCD2 by Western Blot

LIN37 function in DNA end protection is restricted to G0.(A, B) Western blot analysis of indicated proteins in cycling and non-cycling abl pre-B cells (A) or MCF10A cells (B). (C, D) Western blot analysis of indicated proteins in cycling G1 or S/G2/M abl pre-B cells (C) or MCF10A cells (D), isolated by flow cytometric cell sorting based on the PIP-FUCCI reporter. Representative of two independent experiments. Asterisk indicates non-specific recognizing bands. (E, F) Flow cytometric analysis of chromatin-bound RPA and gamma H2AX before and after IR treatment of G1-phase Lig4−/−, Lig4−/−:Trp53bp1−/− and Lig4−/−:Lin37−/− abl pre-B cells (E) or WT, Trp53bp1−/−, and Lin37−/− MCF10A cells (F). Representative of three experiments. IR, ionizing radiation; WT, wild type.Figure 7—source data 1.RNA-Seq result and GO analysis in cycling G1Lig4−/− and Lig4−/−:Lin37−/− abl pre-B cells.GO, gene ontology; RNA-Seq, RNA sequencing.Figure 7—source data 2.GO analysis of genes upregulated in non-cycling G0 and/or cycling G1Lig4−/−:Lin37−/− abl pre-B cells.GO, gene ontology.RNA-Seq result and GO analysis in cycling G1Lig4−/− and Lig4−/−:Lin37−/− abl pre-B cells.GO, gene ontology; RNA-Seq, RNA sequencing.GO analysis of genes upregulated in non-cycling G0 and/or cycling G1Lig4−/−:Lin37−/− abl pre-B cells.GO, gene ontology.Identification of G1-phase cells in proliferating cells.(A, B) Flow cytometric analysis of EdU incorporation and DNA content (7-AAD) of cycling Lig4−/−, Lig4−/−:Trp53bp1−/−, and Lig4−/−:Lin37−/− abl pre-B cells (A) or cycling WT, Trp53bp1−/−, and Lin37−/− MCF10A cells (B). Cells were treated (+EdU) or not treated (−EdU) with EdU and analyses were carried out before or after IR. The percentage of G1-phase cells is shown. IR, ionizing radiation; WT, wild type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34477552), licensed under a CC-BY license. Not internally tested by R&D Systems.