Detection of Human, Mouse, and Rat FBPase 1 by Western Blot.
Western blot shows lysates of MCF‑7 human breast cancer cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat FBPase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7285) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FBPase 1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: FBPase 1
FBPase 1 (Fructose 1,6 bisphosphatase 1 [Fru-1,6-P2ase]; also FBP-1 phosphohydrolase 1) is a 35-37 kDa member of the FBPase Class I family of enzymes. It is widely expressed, being found in cells diverse as monocytes, hepatocytes, pancreatic beta -cells and striated (skeletal plus cardiac) muscle cells. FBPase 1 converts Fru-1,6-P2 into Fru-6-P, a molecule that is subsequently used to generate glucose. Human FBPase 1 is 338 amino acids (aa) in length. It contains one catalytic region (aa 18-332) and an NLS between aa 203-208. FBPase 1 exhibits divalent cation dependency, and exists as a homotetramer within the cell. Full-length human FBPase 1 shares 85% aa sequence identity with mouse FBPase 1.
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