Human/Mouse/Rat FBPase 1 Antibody

Catalog # Availability Size / Price Qty
FBPase 1 in Human Liver Cancer Tissue.
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Product Details

Human/Mouse/Rat FBPase 1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, rat FBPase 1 in Western blots and detects human FBPase 1 in direct ELISAs.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human FBPase 1
Ala2-Gln338 (Arg218Lys)
Accession # P09467
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry FBPase 1 antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

FBPase 1 in Human Liver Cancer Tissue. FBPase 1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Sheep Anti-Human/Mouse/Rat FBPase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7285) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot Detection of Human, Mouse, and Rat FBPase 1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat FBPase 1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat FBPase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7285) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FBPase 1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FBPase 1

FBPase 1 (Fructose 1,6 bisphosphatase 1 [Fru-1,6-P2ase]; also FBP-1 phosphohydrolase 1) is a 35-37 kDa member of the FBPase Class I family of enzymes. It is widely expressed, being found in cells diverse as monocytes, hepatocytes, pancreatic beta -cells and striated (skeletal plus cardiac) muscle cells. FBPase 1 converts Fru-1,6-P2 into Fru-6-P, a molecule that is subsequently used to generate glucose. Human FBPase 1 is 338 amino acids (aa) in length. It contains one catalytic region (aa 18-332) and an NLS between aa 203-208. FBPase 1 exhibits divalent cation dependency, and exists as a homotetramer within the cell. Full-length human FBPase 1 shares 85% aa sequence identity with mouse FBPase 1.

Long Name
Fructose-1,6-bisphosphatase 1
Entrez Gene IDs
2203 (Human); 14121 (Mouse); 24362 (Rat)
Alternate Names
D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; EC 3.1.3; EC; FBP; FBP1; FBPase 1; fructose-1,6-bisphosphatase 1; fructose-bisphosphatase 1; growth-inhibiting protein 17

Product Datasheets


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