Human/Mouse/Rat FBPase 1 Antibody

Catalog # Availability Size / Price Qty
AF7285
AF7285-SP
Detection of Human, Mouse, and Rat FBPase 1 by Western Blot.
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Human/Mouse/Rat FBPase 1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, rat FBPase 1 in Western blots and detects human FBPase 1 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FBPase 1
Ala2-Gln338 (Arg218Lys)
Accession # P09467
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat FBPase 1 by Western Blot. View Larger

Detection of Human, Mouse, and Rat FBPase 1 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat FBPase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7285) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FBPase 1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FBPase  1 in Human Liver Cancer Tissue. View Larger

FBPase 1 in Human Liver Cancer Tissue. FBPase 1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Sheep Anti-Human/Mouse/Rat FBPase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7285) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FBPase 1

FBPase 1 (Fructose 1,6 bisphosphatase 1 [Fru-1,6-P2ase]; also FBP-1 phosphohydrolase 1) is a 35-37 kDa member of the FBPase Class I family of enzymes. It is widely expressed, being found in cells diverse as monocytes, hepatocytes, pancreatic beta -cells and striated (skeletal plus cardiac) muscle cells. FBPase 1 converts Fru-1,6-P2 into Fru-6-P, a molecule that is subsequently used to generate glucose. Human FBPase 1 is 338 amino acids (aa) in length. It contains one catalytic region (aa 18-332) and an NLS between aa 203-208. FBPase 1 exhibits divalent cation dependency, and exists as a homotetramer within the cell. Full-length human FBPase 1 shares 85% aa sequence identity with mouse FBPase 1.

Long Name
Fructose-1,6-bisphosphatase 1
Entrez Gene IDs
2203 (Human); 14121 (Mouse); 24362 (Rat)
Alternate Names
D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; EC 3.1.3; EC 3.1.3.11; FBP; FBP1; FBPase 1; fructose-1,6-bisphosphatase 1; fructose-bisphosphatase 1; growth-inhibiting protein 17

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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