Human/Mouse/Rat Fgr Antibody Summary
Accession # P09769
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Rat Fgr by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat Fgr Monoclonal Antibody (Catalog # MAB3207) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fgr at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fgr is the cellular homolog of the oncogenic protein encoded by the Gardner-Rasheed feline sarcoma virus. A member of the Src family of non‑receptor tyrosine kinases, Fgr is predominantly expressed in neutrophils, macrophages, monocytes, and natural killer cells. Mice lacking Fgr fail to recruit eosinophils to the lung during allergic airway inflammation.
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