Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 349316
View all Fgr Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Fgr
Met1-Phe82
Accession # P09769

Specificity

Detects endogenous human, mouse, and rat Fgr in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat Fgr Antibody

Detection of Human and Rat Fgr antibody by Western Blot.

Detection of Human and Rat Fgr by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat Fgr Monoclonal Antibody (Catalog # MAB3207) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fgr at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human/Mouse/Rat Fgr Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: NRK rat normal kidney cell line, Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB3207 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fgr

Fgr is the cellular homolog of the oncogenic protein encoded by the Gardner-Rasheed feline sarcoma virus. A member of the Src family of non‑receptor tyrosine kinases, Fgr is predominantly expressed in neutrophils, macrophages, monocytes, and natural killer cells. Mice lacking Fgr fail to recruit eosinophils to the lung during allergic airway inflammation.

Long Name

Gardner-Rasheed Feline Sarcoma Viral Oncogene Homolog

Alternate Names

Src2

Entrez Gene IDs

2268 (Human); 14191 (Mouse); 79113 (Rat)

Gene Symbol

FGR

UniProt

P09769

Additional Fgr Products

Product Documents for Human/Mouse/Rat Fgr Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Fgr Antibody

For research use only

Related Research Areas

Intracellular Kinases

Customer Reviews for Human/Mouse/Rat Fgr Antibody (1)

5 out of 5
1 Customer Rating
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  • Human/Mouse/Rat Fgr Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lymphoma cell line
    Species: Human
    Verified Customer | Posted 01/16/2022
    Human/Mouse/Rat Fgr Antibody MAB3207

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