Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 494310
View all HSP20/HSPB6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSP20/HSPB6
Met1-Lys160
Accession # O14558

Specificity

Detects human, mouse, and rat HSP20/HSPB6 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat HSP20/HSPB6 Antibody

Detection of Human/Mouse/Rat HSP20/HSPB6 antibody by Western Blot.

Detection of Human/Mouse/Rat HSP20/HSPB6 by Western Blot.

Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HSP20/HSPB6 Monoclonal Antibody (Catalog # MAB4200) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP20/HSPB6 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human HSP20/HSPB6 by Simple WesternTM.

Simple Western shows lysates of human heart tissue, loaded at 0.5 mg/ml. A specific band was detected for HSP20/HSPB6 at approximately 26 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat HSP20/HSPB6 Monoclonal Antibody (Catalog # MAB4200). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human/Mouse/Rat HSP20/HSPB6 Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Human heart tissue

Western Blot

1 µg/mL
Sample: Human heart tissue, mouse heart tissue, and rat heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Tween® 20 with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSP20/HSPB6

Heat shock protein 20 kDa (HSP20), also known as heat shock protein, alpha-crystallin-related, B6 (HSPB6), is a 160 amino acid, 20 kDa member of the small heat shock protein family, which also includes HSP27, HSP25, and the alpha -crystallins. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSPs are ubiquitously expressed in all organisms. Many HSPs are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Although HSP20 expression does not increase in response to heat stress, HSP20 does translocate from the cytosol to the nucleus during heat stress. The highest levels of HSP20 are found in skeletal, smooth and cardiac muscle. HSP20 may also be secreted into plasma. Human HSP20 shares 88% and 90% amino acid identity with mouse and rat HSP20, respectively.

Long Name

Heat Shock Protein 20

Alternate Names

HSPB6

Entrez Gene IDs

126393 (Human); 243912 (Mouse); 192245 (Rat)

Gene Symbol

HSPB6

UniProt

O14558 (Human)

Additional HSP20/HSPB6 Products

Product Documents for Human/Mouse/Rat HSP20/HSPB6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat HSP20/HSPB6 Antibody

For research use only

Citations for Human/Mouse/Rat HSP20/HSPB6 Antibody

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