Detection of Human/Mouse/Rat HSP20/HSPB6 by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HSP20/HSPB6 Monoclonal Antibody (Catalog # MAB4200) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP20/HSPB6 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Heat shock protein 20 kDa (HSP20), also known as heat shock protein, alpha-crystallin-related, B6 (HSPB6), is a 160 amino acid, 20 kDa member of the small heat shock protein family, which also includes HSP27, HSP25, and the alpha -crystallins. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSPs are ubiquitously expressed in all organisms. Many HSPs are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Although HSP20 expression does not increase in response to heat stress, HSP20 does translocate from the cytosol to the nucleus during heat stress. The highest levels of HSP20 are found in skeletal, smooth and cardiac muscle. HSP20 may also be secreted into plasma. Human HSP20 shares 88% and 90% amino acid identity with mouse and rat HSP20, respectively.
Heat Shock Protein 20
Entrez Gene IDs:
126393 (Human); 243912 (Mouse); 192245 (Rat)
FLJ32389; Heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein, alpha-crystallin-related, B6; Hsp20; HSPB6
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