HSP20/HSPB6 Antibody (494310)

R&D Systems | Catalog # MAB4200

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 494310
View all HSP20/HSPB6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSP20/HSPB6
Met1-Lys160
Accession # O14558

Specificity

Detects human, mouse, and rat HSP20/HSPB6 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HSP20/HSPB6 Antibody (494310)

Detection of Human/Mouse/Rat HSP20/HSPB6 antibody by Western Blot.

Detection of Human/Mouse/Rat HSP20/HSPB6 by Western Blot.

Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HSP20/HSPB6 Monoclonal Antibody (Catalog # MAB4200) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP20/HSPB6 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human HSP20/HSPB6 by Simple WesternTM.

Simple Western shows lysates of human heart tissue, loaded at 0.5 mg/ml. A specific band was detected for HSP20/HSPB6 at approximately 26 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat HSP20/HSPB6 Monoclonal Antibody (Catalog # MAB4200). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Detection of HSP20/HSPB6 by Western Blot

Detection of HSP20/HSPB6 by Western Blot

Small heat shock proteins enrich at mitochondria after heat shock in multiple human and mouse cell lines.a, Mitochondria were isolated and compared to the cytosol and the NP-40-soluble whole cell lysate (WCL) from: HEK293T (human embryonic kidney cells), NSC-34 (motor neuron-like cells), Neuro-2A (neuronal crest-derived), SH-SY5Y (neuroblastoma cells), COS1 (African green monkey derived kidney cells), A498 (renal carcinoma cells), and healthy volunteer-derived human lymphoblasts. Cells were either left untreated or heat shocked (42 °C) for 1 h. Samples were analyzed by SDS-PAGE followed by immunoblotting using antibodies against sHSPs, Tubulin (cytosol), and VDAC1 (mitochondria). b, Mitochondria were isolated from C2C12 cells after heat shock (42 °C) for the indicated times. Samples were analyzed by SDS-PAGE followed by immunoblotting using antibodies against sHSPs or VDAC1 (loading control). Results are representative of two replicates (a,b). Unprocessed blots are available in source data.Source data Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36690850), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for HSP20/HSPB6 Antibody (494310)

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Human heart tissue

Western Blot

1 µg/mL
Sample: Human heart tissue, mouse heart tissue, and rat heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Tween® 20 with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSP20/HSPB6

Heat shock protein 20 kDa (HSP20), also known as heat shock protein, alpha-crystallin-related, B6 (HSPB6), is a 160 amino acid, 20 kDa member of the small heat shock protein family, which also includes HSP27, HSP25, and the alpha -crystallins. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSPs are ubiquitously expressed in all organisms. Many HSPs are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Although HSP20 expression does not increase in response to heat stress, HSP20 does translocate from the cytosol to the nucleus during heat stress. The highest levels of HSP20 are found in skeletal, smooth and cardiac muscle. HSP20 may also be secreted into plasma. Human HSP20 shares 88% and 90% amino acid identity with mouse and rat HSP20, respectively.

Long Name

Heat Shock Protein 20

Alternate Names

HSPB6

Entrez Gene IDs

126393 (Human); 243912 (Mouse); 192245 (Rat)

Gene Symbol

HSPB6

UniProt

O14558 (Human)

Additional HSP20/HSPB6 Products

Product Documents for HSP20/HSPB6 Antibody (494310)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for HSP20/HSPB6 Antibody (494310)

For research use only

Citations for HSP20/HSPB6 Antibody (494310)

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