IMP Dehydrogenase 2/IMPDH2 Antibody (911425)
R&D Systems | Catalog # MAB8349
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 911425
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2
Met1-Phe514
Accession # P12268
Met1-Phe514
Accession # P12268
Specificity
Detects human Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 in direct ELISAs. Detects human, mouse and rat IMPDH2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for IMP Dehydrogenase 2/IMPDH2 Antibody (911425)
Detection of Human, Mouse, and Rat Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, BaF3 mouse pro-B cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 Monoclonal Antibody (Catalog # MAB8349) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 by Simple WesternTM.
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 at approximately 57 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 Monoclonal Antibody (Catalog # MAB8349). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for IMP Dehydrogenase 2/IMPDH2 Antibody (911425)
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line
Western Blot
0.5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, BaF3 mouse pro-B cell line, and Nb2‑11 rat lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, BaF3 mouse pro-B cell line, and Nb2‑11 rat lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IMP Dehydrogenase 2/IMPDH2
Long Name
Inosine 5'-Monophosphate Dehydrogenase 2
Alternate Names
IMDH2, IMP Oxireductase 2, IMPD2, IMPDH2
Gene Symbol
IMPDH2
UniProt
Additional IMP Dehydrogenase 2/IMPDH2 Products
Product Documents for IMP Dehydrogenase 2/IMPDH2 Antibody (911425)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for IMP Dehydrogenase 2/IMPDH2 Antibody (911425)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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