Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 911425
View all IMP Dehydrogenase 2/IMPDH2 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2
Met1-Phe514
Accession # P12268

Specificity

Detects human Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 in direct ELISAs. Detects human, mouse and rat IMPDH2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Detection of Human, Mouse, and Rat Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 antibody by Western Blot.

Detection of Human, Mouse, and Rat Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, BaF3 mouse pro-B cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 Monoclonal Antibody (Catalog # MAB8349) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 antibody by Simple WesternTM.

Detection of Human Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 by Simple WesternTM.

Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 at approximately 57 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 Monoclonal Antibody (Catalog # MAB8349). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line

Western Blot

0.5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, BaF3 mouse pro-B cell line, and Nb2‑11 rat lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IMP Dehydrogenase 2/IMPDH2

IMP Dehydrogenase 2 (IMPDH2), also known as type II Inosine 5'-Monophosphate Dehydrogenase and IMPD2, is the rate-limiting enzyme in de novo guanine nucleotide biosynthesis. Along with closely related enzyme IMPDH1, IMPDH2 catalyzes the conversion of Inosine 5’-Phosphate (IMP) to Xanthosine 5’-Phosphate (XMP), and is responsible for maintaining the cellular guanine deoxy- and ribonucleotide pools required for DNA and RNA synthesis, respectively. IMPDH2 regulation is associated with cellular proliferation, transformation and differentiation, and its levels are selectively upregulated in neoplastic and replicating cells. Therefore, it has been evaluated as a target for anti-tumor, anti-infective and immunosuppressive drugs.  Human IMPDH2 is a 56 kDa protein that shares 84% amino acid (aa) identity with human IMPDH1, and 98% aa identity with mouse and rat IMPDH2.

Long Name

Inosine 5'-Monophosphate Dehydrogenase 2

Alternate Names

IMDH2, IMP Oxireductase 2, IMPD2, IMPDH2

Entrez Gene IDs

3615 (Human); 23918 (Mouse); 301005 (Rat)

Gene Symbol

IMPDH2

UniProt

P12268

Additional IMP Dehydrogenase 2/IMPDH2 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices

For research use only

Related Research Areas

Redox Enzymes

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