Human/Mouse/Rat Latrophilin 3/LPHN3 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human Latrophilin 3/LPHN3 in direct ELISAs and human, mouse, and rat Latrophilin 3/LPHN3 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 801518
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Latrophilin 3/LPHN3
    Met1-Arg813
    Accession # Q9HAR2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    20 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Latrophilin 3/LPHN3 by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Latrophilin 3/LPHN3 Monoclonal Antibody (Catalog # MAB5916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Latrophilin 3/LPHN3 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Rat Latrophilin 3/LPHN3 by Simple WesternTM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue and rat brain tissue, loaded at 0.5 mg/mL. A specific band was detected for Latrophilin 3/LPHN3 at approximately 109 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Latrophilin 3/LPHN3 Monoclonal Antibody (Catalog # MAB5916). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Latrophilin 3/LPHN3
LPHN3 (Latrophilin 3; also CIRL3, CL3 and LEC3) is a (presumably) 220 kDa member of the LN-TM7 subfamily, GPCR 2 family of molecules. It appears to have a restricted expression pattern, being limited to brain and adrenal gland. Although it is related to the black widow toxin receptor CIRL1, it does not serve as a toxin receptor. Mature human Latrophilin 3 is a 7-TM glycoprotein that is 1428 amino acids (aa) in length. Post-translational processing cleaves the molecule into a 120 kDa ECD (aa 20-841) and a noncovalently-associated 85 kDa 7-TM C-terminus (aa 842-1447). The ECD is modular and contains a SUEL-like lectin domain (aa 35-124), an Olf region (aa 134-393) and GPS domain (aa 802-853). There are multiple splice events which, in the ECD, include a two aa substitution for aa 127-131, a 13 aa insertion after Lys623, and a 39 aa substitution for aa 668-1447. Over aa 20-832, human Latrophilin 3 shares 98% aa identity with mouse Latrophilin 3.
  • Entrez Gene IDs:
    23284 (Human); 319387 (Mouse); 170641 (Rat)
  • Alternate Names:
    CIRL3; Latrophilin 3; latrophilin homolog 3 (cow); LEC3; Lectomedin-3; LPHN3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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