Detection of Human, Mouse, and Rat Latrophilin 3/LPHN3 by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Latrophilin 3/LPHN3 Monoclonal Antibody (Catalog # MAB5916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Latrophilin 3/LPHN3 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Rat Latrophilin 3/LPHN3 by Simple WesternTM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue and rat brain tissue, loaded at 0.5 mg/mL. A specific band was detected for Latrophilin 3/LPHN3 at approximately 109 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Latrophilin 3/LPHN3 Monoclonal Antibody (Catalog # MAB5916). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Latrophilin 3/LPHN3
LPHN3 (Latrophilin 3; also CIRL3, CL3 and LEC3) is a (presumably)
220 kDa member of the LN-TM7 subfamily, GPCR 2 family of molecules. It appears to have a restricted expression
pattern, being limited to brain and adrenal gland. Although it is related to the black widow
toxin receptor CIRL1, it does not serve as a toxin receptor. Mature human Latrophilin 3 is a 7-TM glycoprotein
that is 1428 amino acids (aa) in length. Post-translational processing cleaves the
molecule into a 120 kDa ECD (aa 20-841) and a noncovalently-associated 85 kDa
7-TM C-terminus (aa 842-1447). The ECD
is modular and contains a SUEL-like lectin domain (aa 35-124), an Olf region
(aa 134-393) and GPS domain (aa 802-853). There are multiple splice events which, in the ECD, include a two aa
substitution for aa 127-131, a 13 aa insertion after Lys623, and a 39 aa
substitution for aa 668-1447. Over aa 20-832, human Latrophilin 3 shares 98% aa identity with mouse Latrophilin 3.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.