Latrophilin 3/LPHN3 Antibody (801518)

R&D Systems | Catalog # MAB5916

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 801518
View all Latrophilin 3/LPHN3 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Latrophilin 3/LPHN3
Met1-Arg813
Accession # Q9HAR2

Specificity

Detects human Latrophilin 3/LPHN3 in direct ELISAs and human, mouse, and rat Latrophilin 3/LPHN3 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Latrophilin 3/LPHN3 Antibody (801518)

Detection of Human, Mouse, and Rat Latrophilin 3/LPHN3 antibody by Western Blot.

Detection of Human, Mouse, and Rat Latrophilin 3/LPHN3 by Western Blot.

Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Latrophilin 3/LPHN3 Monoclonal Antibody (Catalog # MAB5916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Latrophilin 3/LPHN3 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Rat Latrophilin 3/LPHN3 antibody by Simple WesternTM.

Detection of Human and Rat Latrophilin 3/LPHN3 by Simple WesternTM.

Simple Western lane view shows lysates of human brain (hypothalamus) tissue and rat brain tissue, loaded at 0.5 mg/mL. A specific band was detected for Latrophilin 3/LPHN3 at approximately 109 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Latrophilin 3/LPHN3 Monoclonal Antibody (Catalog # MAB5916). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Latrophilin 3/LPHN3 Antibody (801518)

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Human brain (hypothalamus) tissue and rat brain tissue

Western Blot

2 µg/mL
Sample: Human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and rat brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Latrophilin 3/LPHN3

LPHN3 (Latrophilin 3; also CIRL3, CL3 and LEC3) is a (presumably) 220 kDa member of the LN-TM7 subfamily, GPCR 2 family of molecules. It appears to have a restricted expression pattern, being limited to brain and adrenal gland. Although it is related to the black widow toxin receptor CIRL1, it does not serve as a toxin receptor. Mature human Latrophilin 3 is a 7-TM glycoprotein that is 1428 amino acids (aa) in length. Post-translational processing cleaves the molecule into a 120 kDa ECD (aa 20-841) and a noncovalently-associated 85 kDa 7-TM C-terminus (aa 842-1447). The ECD is modular and contains a SUEL-like lectin domain (aa 35-124), an Olf region (aa 134-393) and GPS domain (aa 802-853). There are multiple splice events which, in the ECD, include a two aa substitution for aa 127-131, a 13 aa insertion after Lys623, and a 39 aa substitution for aa 668-1447. Over aa 20-832, human Latrophilin 3 shares 98% aa identity with mouse Latrophilin 3.

Alternate Names

CIRL3, LEC3, Lectomedin-3, LPHN3

Entrez Gene IDs

23284 (Human); 319387 (Mouse); 170641 (Rat)

Gene Symbol

ADGRL3

UniProt

Q9HAR2 (Human)

Additional Latrophilin 3/LPHN3 Products

Product Documents for Latrophilin 3/LPHN3 Antibody (801518)

Certificate of Analysis

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Product Specific Notices for Latrophilin 3/LPHN3 Antibody (801518)

For research use only

Citations for Latrophilin 3/LPHN3 Antibody (801518)

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