Human/Mouse/Rat Ninjurin‑2 Antibody

Detection of Ninjurin-2 by Western Blot

NINJ2 expression in ECF-R gastric cancer cells. (A) Representative IC50 values for parent (WT) and ECF-resistant (ECF-R) MKN-74 and SNU-484 cells. The IC50 values are given in an equation using a four-parameter logistic curve. (B) Parent and ECF-R MKN-74 cells were individually transplanted into nude mice and left until the tumor reached a volume of 100 mm3. The tumor-bearing mice were given ECF, and the tumor volume was measured. *p < 0.05 versus parent at each time point. (C) (Left) Venn diagram showing top genes with 15-fold changes in the RNA-sequencing of WT and ECF-R genes related to “Integral component of plasma membrane (GO:0005887)” and “Cell adhesion (GO:0007155).” (Right) Heatmap showing the gene expression level associated with resistance and the two gene ontology categories. (D) RGV analysis for the human NINJ2 isoform based on RNA-sequencing of WT and ECF-R (hg19 base) cells. (E) mRNA levels of NINJ2 was analyzed by quantitative real-time PCR in parent and ECF-R cells from the MKN-28/74, MKN-74, MKN-45, SNU-484, SNU-520, and SNU-688 lines. (F) Protein levels of NINJ2 in parent and ECF-R MKN-74 and SNU-484 cells were determined by Western blot analysis. ECF, epirubicin, cisplatin, and 5-fluorouracil; ECF-R, ECF-resistance. Data are presented as mean ± SD Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40518514), licensed under a CC-BY license. Not internally tested by R&D Systems.