|Detection of Human Ninjurin‑2 by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, mouse spleen tissue and rat thymus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Ninjurin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5056) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ninjurin‑2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
Ninjurin-2 (nerve injury-induced protein 2) is a 20‑22 kDa member of the Ninjurin family of transmembrane (TM) proteins. It is expressed by multiple cell types, including Schwann cells, myenteric plexus and sensory neurons, and lymphocytes and participates in intercellular homophilic binding. Human Ninjurin-2 is 142 amino acids (aa) in length. It has an unusual membrane orientation. There is a 65 aa N-terminal extracellular domain (ECD) (aa 1‑65) that contains one phoshorylation site at Ser3, followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 128‑142). One potential alternate start site exists 46 aa upstream of the standard form start site. Over aa 1‑65, human Ninjurin-2 is 71% aa identical to mouse Ninjurin-2.
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