Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 783403
View all Nucleoporin NUP85 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Nucleoporin NUP85
Met1-Ser656
Accession # Q9BW27

Specificity

Detects human Nucleoporin NUP85 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat Nucleoporin NUP85 Antibody

Detection of Human, Mouse, and Rat Nucleoporin NUP85 antibody by Western Blot.

Detection of Human, Mouse, and Rat Nucleoporin NUP85 by Western Blot.

Western blot shows lysates of NR8383 rat alveolar macrophage cell line, K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, M1 mouse myeloid leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat Nucleoporin NUP85 Monoclonal Antibody (Catalog # MAB5976) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nucleoporin NUP85 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Nucleoporin NUP85 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: NR8383 rat alveolar macrophage cell line, K562 human chronic myelogenous leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, M1 mouse myeloid leukemia cell line, and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nucleoporin NUP85

NUP85 (Nucleoporin 85; also Nup75/nucleoporin 75 and FROUNT) is a 70‑72 kDa member of the nucleoporin Nup85 family of proteins. It is one of nine members of the Nup107-160 subcomplex that performs at least two functions in the cell. First, it serves as an assembly point for nuclear pore formation, and may stabilize the bend that normally exists between the inner and outer nuclear membranes. Second, it appears to be quite stable, and during mitosis, promotes mitotic spindle assembly. Human NUP85 is 656 amino acids (aa) in length. It is an alpha -helical protein with no obvious domain(s) or structural motif. Phosphorylation on Ser233, however, may regulate the overall interaction of the NUP107‑160 subcomplex with the much larger nuclear pore. There one potential splice form that shows an alternative start site at Met47. Full-length NUP85 shares 92% aa identity with mouse NUP85.

Long Name

Nuclear Pore Complex Protein Nup85

Alternate Names

FROUNT, Nup75, NUP85, PCNT1, Pericentrin-1

Entrez Gene IDs

79902 (Human); 445007 (Mouse); 287830 (Rat)

Gene Symbol

NUP85

UniProt

Q9BW27 (Human)

Additional Nucleoporin NUP85 Products

Product Documents for Human/Mouse/Rat Nucleoporin NUP85 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Nucleoporin NUP85 Antibody

For research use only

Related Research Areas

Monocyte Markers

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