Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA

R&D Systems | Catalog # DYC1387B-2

R&D Systems
100% Guarantee

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

156-10000 pg/mL

Sample Type

Cell Lysates

Reactivity

Human, Mouse, Rat

Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot
View all JNK ELISA Kits »

Product Summary for Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat JNK in cell lysates. An immobilized capture antibody specific for JNK binds both phosphorylated and unphosphorylated JNK. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

Cell lysates (100 µL)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA

Standard Curve

Kit Contents for Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS:

(Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: JNK

Members of the MAPK family, the c-Jun N-terminal kinases (JNKs) are activated by environmental stresses and inflammatory cytokines. Ten JNK isoforms are created by alternative splicing of mRNA transcripts derived from three genes: JNK1, JNK2, and JNK3. All JNKs are activated by dual phosphorylation; at T183/Y185 for JNK1 and 2, and T221/Y223 for JNK3. Activated JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.

Long Name

C-Jun N-terminal Kinase

Alternate Names

c-Jun N-terminal kinase 1, EC 2.7.11, EC 2.7.11.24, JNK1 alpha protein kinase, JNK1 beta protein kinase, JNK1JNK1A2, JNK-46, JUN N-terminal kinase, MAP kinase 8, MAPK 8, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2, PRKM8JNK, protein kinase JNK1, SAPK1JNK21B1/2, Stress-activated protein kinase 1, Stress-activated protein kinase JNK1

Additional JNK Products

Product Documents for Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA

For research use only

Citations for Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA

Customer Reviews for Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA and earn rewards!

Have you used Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits