Detection of Human/Mouse/Rat PKA C by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat PKA C Pan Specific Monoclonal Antibody (Catalog # MAB4175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (5 ng/lane) were included. A specific band for PKA C (pan) was detected at approximately 39 - 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKA C (pan)
The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.
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