Detection of Human/Mouse/Rat PKC epsilon by Western Blot. Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKC epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5134) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKC epsilon at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PKC epsilon in Human Lung Cancer Tissue. PKC epsilon was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Human/Mouse/Rat PKC epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5134) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC epsilon
PKC epsilon (protein kinase C-epsilon) is an 87 kDa member of the novel PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a widely-expressed Ca++‑insensitive, phospholipid-dependent enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC epsilon is 737 amino acids (aa) in length. It contains two general regions: a non-Ca++-binding plus lipid binding regulatory region (aa’s 1‑99 and 169‑292, respectively), and an ATP-binding catalytic domain (aa 408‑668). Phosphorylation of PKC epsilon on Thr566, Ser368 and Ser729 activates its enzymatic activity and increases its molecular weight to 92 kDa. One potential splice form shows a 114 aa substitution for aa 118‑737. Over aa 580‑737, human PKC epsilon is 98% and 99% aa identical to mouse and canine PKC epsilon, respectively.
Protein Kinase C epsilon
Entrez Gene IDs:
5581 (Human); 18754 (Mouse); 29340 (Rat)
EC 2.7.11; EC 220.127.116.11; MGC125656; nPKC-epsilon; PKCE; PKCEMGC125657; PRKCE; protein kinase C epsilon type; protein kinase C, epsilon
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