Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PKC iota/lambda/zeta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKC iota
Ile455-Val596
Accession # P41743

Specificity

Detects human, mouse and rat PKC iota / lambda / zeta in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PKC iota / lambda / zeta Antibody

Detection of Human, Mouse, and Rat PKC?/?/? antibody by Western Blot.

Detection of Human, Mouse, and Rat PKC iota / lambda / zeta by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, DU145 human prostate carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat PKC iota/lambda/zeta Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, recombinant human PKC iota, PKC zeta, and PKC alpha (2 ng/lane) were included. Specific bands were detected at approximately 22 kDa for recombinant PKC iota/zeta and ~80 kDa for natural PKC iota/zeta (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PKC?/?/? antibody in Human Pancreas by Immunohistochemistry (IHC-P).

PKC iota / lambda / zeta in Human Pancreas.

PKC iota/lambda/zeta was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human/Mouse/Rat PKC iota/lambda/zeta Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples.

Detection of Human PKC iota / lambda / zeta by Simple WesternTM.

Simple Western shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for PKC iota / lambda / zeta at approximately 80 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat PKC iota / lambda / zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human/Mouse/Rat PKC iota / lambda / zeta Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Simple Western

10 µg/mL
Sample: A431 human epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, DU145 human prostate carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 1 review rated 4 using AF4465 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKC iota/lambda/zeta

Members of the Protein Kinase C (PKC) family are serine/threonine protein kinases that play a key regulatory role in a number of cellular functions including cell growth and differentiation, hormone secretion, and gene expression. Multiple genes and alternative splicing result in three subfamilies, which differ in their co‑factor requirements: conventional PKC isoforms ( alpha, beta Ι, beta ΙΙ, and gamma ) which require calcium and phosphatidylserine (PS), diacylglycerol (DAG) or phorbol esters for activation; novel isoforms ( delta, epsilon, eta, and theta ), which are calcium-independent but are still regulated by PS, DAG, or phorbol esters; and atypical isoforms ( iota, lambda, and zeta ), which are calcium-independent and do not require PS, DAG, or phorbol esters for activation. PKC iota has 72% overall identity to PKC zeta. Atypical PKCs have been shown to serve as a convergent downstream target for the PI 3-kinase and TC10 signaling pathways. Stimulation of atypical PKCs by TNF-alpha has been shown to be required for
NF‑ kappa B activation. Furthermore, insulin-stimulated atypical PKC activation has been directly implicated in the translocation of GLUT4 and glucose uptake in adipocytes.

Long Name

Protein Kinase C iota/lambda/zeta

UniProt

P41743

Additional PKC iota/lambda/zeta Products

Product Documents for Human/Mouse/Rat PKC iota / lambda / zeta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat PKC iota / lambda / zeta Antibody

For research use only

Related Research Areas

Diabetes
Intracellular Kinases
PDGF Family

Citations for Human/Mouse/Rat PKC iota / lambda / zeta Antibody

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  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Hippocampus
    Species: Mouse
    Verified Customer | Posted 07/31/2015
    Human/Mouse/Rat PKC iota / lambda / zeta Antibody AF4465

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Protocols

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FAQs for Human/Mouse/Rat PKC iota / lambda / zeta Antibody

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  • Q: Does this antibody work as an IP antibody to pull-down PKCiota/lambda and their phosphorylated form?

    A: For product AF4465, this item has not yet been tested in an immunoprecipitation assay. This antibody is manufactured by our sister company R&D Systems and has only been tested so far in western blot and IHC per the R&D Systems site. The immunogen of the antibody is found between aa 455-596 of the human Protein kinase C iota type protein. This sequence encompasses one phosphorylation site (aa 564), and therefore has the potential to recognize the phosphorylated and non-phosphorylated forms of the protein; with exception of p Thr 564. This, of course, is hypothetical as the antibody has not yet been tested against the phosphorylated forms of the proteins.

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