Human/Mouse/Rat Plexin A4 Antibody

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Detection of Human, Mouse, and Rat Plexin A4 by Western Blot.
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Product Details
Citations (2)
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Human/Mouse/Rat Plexin A4 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human Plexin A4 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) Plexin A1 is observed. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human Plexin B1, B2, B3, C1, D1, rmPlexin A2, and A3 is observed.
Monoclonal Mouse IgG1 Clone # 707201
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Plexin A4
Accession # Q9HCM2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
Human Brain cortex and Mouse Brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Plexin A4 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Plexin A4 by Western Blot. Western blot shows lysates of human brain (cortex) tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Plexin A4 Monoclonal Antibody (Catalog # MAB5856) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Plexin A4 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human/Mouse/Rat Plexin A4 by Simple WesternTM. Simple Western lane view shows lysates of Human Brain cortex and Mouse Brain, loaded at 0.2 mg/mL. A specific band was detected for Plexin A4 at approximately 230 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Plexin A4 Monoclonal Antibody (Catalog # MAB5856). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin A4

Plexin A4 is a 220‑230 kDa member of the plexin A subfamily, plexin family of proteins (1). It is found on sensory, autonomic and motor neurons and oligodendrocytes, plus T cells and dendritic cells (1‑8). Mature human Plexin A4 is an 1871 amino acid (aa) type I transmembrane glycoprotein with a 23 aa signal sequence, a 1214 aa extracellular domain (ECD), and a 636 aa cytoplasmic region. The ECD contains one Sema-domain (aa 51‑482), three PSI domains (aa 509‑856) and four IPT regions (aa 858‑1230) that contain a phosphoserine at aa 946 (1). Of three isoform variants, one shows a 65 aa substitution for aa 458‑1894, a second shows an 80 aa substitution for aa 1292‑1894, and a third shows the just mentioned 80 aa substitution coupled to a 14 aa substitution for aa 1‑535 (9). The human Plexin A4 ECD shares 97% aa identity with mouse, equine, canine, and bovine Plexin A4. Full‑length Plexin A4 also shares 67% aa identity with the most related family member, Plexin A2. Plexin A4 regulates cell migration, activation and axon guidance via repulsion (1‑5). It serves as a receptor for transmembrane semaphorins, Sema6A and 6B, and as a coreceptor with neuropilin-1 for the secreted semaphorin, Sema3A (1‑8). During development, it plays a role in nerve migration and midline crossing and down‑regulates dendrite formation (2‑8). It is often co‑expressed with Plexin A3, which can also engage class 6 semaphorins but prefers Sema3F/ neuropilin‑2 to Sema3A/neuropilin-1 (3, 8). Thus, Plexins A3 and A4 are redundant in some functions, but unique in others. In T cells, Plexin A4 engages Sema3A and negatively regulates TCR signals (6).

  1. Suto, F. et al. (2003) Mech. Dev. 120:385.
  2. Suto, F. et al. (2005) J. Neurosci. 25:3628.
  3. Faulkner, R.L. et al. (2008) Neural Dev. 3:21.
  4. Waimey, K.E. et al. (2008) Dev. Biol. 315:448.
  5. Runker, A.E. et al. (2008) Neural Dev. 3:34.
  6. Yamamoto, M. et al. (2008) Int. Immunol. 20:413.
  7. Okada, A. et al. (2007) Biochem. Biophys. Res. Commun. 352:158.
  8. Yaron, A. et al. (2005) Neuron 45:513.
  9. Protein Accession # NP_861440, EAW83796, EAL24077.
Entrez Gene IDs
91584 (Human); 243743 (Mouse); 312213 (Rat)
Alternate Names
A; DKFZp434G0625; FAYV2820; FLJ35026; FLJ38287; KIAA1550DKFZp566O0546; PLEXA4; Plexin A4; plexin A4, B; plexin-A4; PLXNA4; PLXNA4A; PLXNA4B; PRO34003

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Citations for Human/Mouse/Rat Plexin A4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Semaphorin3A/PlexinA3 association with the Scribble scaffold for cGMP increase is required for apical dendrite development
    Authors: J Szczurkows, A Guo, J Martin, SI Lee, E Martinez, CT Chien, TA Khan, R Singh, D Dadson, TS Tran, S Pautot, M Shelly
    Cell Reports, 2022-03-15;38(11):110483.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: ICC, IHC
  2. B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex
    Authors: JR Podojil, MY Chiang, I Ifergan, R Copeland, LN Liu, S Maloveste, S Langermann, D Liebenson, R Balabanov, H Chi, L Chen, DAA Vignali, SD Miller
    J. Immunol., 2018-06-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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