Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 257812
View all SHIP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse SHIP
Glu874-Thr941
Accession # Q9ES52

Specificity

Detects human, mouse, and rat SHIP in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat SHIP Antibody

Detection of Human SHIP antibody by Western Blot.

Detection of Human SHIP by Western Blot.

Western blot shows lysates of TF-1 human erythroleukemic cell line. Gels were loaded with 10 µg (lane 1), 20 µg (lane 2), and 40 µg (lane 3). PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band for SHIP was detected at approximately 148 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
SHIP antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

SHIP in Mouse Splenocytes.

SHIP was detected in immersion fixed mouse splenocytes using Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
SHIP Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

SHIP Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

U-937 WT and SHIP KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) followed by incubation with a goat anti-rat Alexa-fluor 555 coupled secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody concentration used: 1 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Human/Mouse/Rat SHIP Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Knockout Validated

SHIP is specifically detected in U-937 parental cell line but is not detectable in SHIP knockout U-937 cell line.

Western Blot

1 µg/mL
Sample: TF‑1 human erythroleukemic cell line

Reviewed Applications

Read 3 reviews rated 3.7 using MAB2317 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SHIP

SH2-containing inositol phosphatase (SHIP), also known as INPP5D, is a negative regulator of signal transduction in hematopoietic cells. Targeted disruption of SHIP in mice leads to a myeloproliferative disorder. Several laboratories have demonstrated the presence of multiple forms of SHIP, including 145 kDa, 135 kDa, and
C‑terminal truncated forms at 125 kDa and 110 kDa in some cell types.

Long Name

SH2-containing Inositol Phosphatase

Alternate Names

INPP5D

Entrez Gene IDs

3635 (Human); 16331 (Mouse); 54259 (Rat)

Gene Symbol

INPP5D

UniProt

Q9ES52

Additional SHIP Products

Product Documents for Human/Mouse/Rat SHIP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat SHIP Antibody

For research use only

Customer Reviews for Human/Mouse/Rat SHIP Antibody (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse/Rat SHIP Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human/Mouse/Rat SHIP Antibody MAB2317
  • Human/Mouse/Rat SHIP Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/09/2019
  • Human/Mouse/Rat SHIP Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: Platelet-poor EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018

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Protocols

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