Human/Mouse/Rat Syntaxin 1B Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human Syntaxin 1B in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Syntaxin 1A and 1B1 is observed, and no cross-reactivity with rhSyntaxin 5, 6, 7, 8, 12, or 16 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 676204
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Syntaxin 1B
    Lys2-Lys264
    Accession # P61266
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Syntaxin 1B by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, CCD‑1070Sk human foreskin fibroblast cell line, Neuro‑2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Syntaxin 1B Monoclonal Antibody (Catalog # MAB6848) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Syntaxin 1B at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syntaxin 1B
Syntaxin 1B/STX1B is an approximately 33 kDa member of the Syntaxin family.  It is a single-pass type IV transmembrane protein that is potentially involved in the docking of synaptic vesicles at presynaptic active zones. The cytoplasmic domain of Syntaxin 1B contains a coiled-coil Syntaxin domain (aa 29-180) which is implicated in the docking of synaptic vesicles with the presynaptic plasma membrane and a t-SNARE coiled coil domain (aa 191-253). Within the cytoplasmic domain, human Syntaxin 1B shares 100% aa sequence identity with mouse and rat Syntaxin 1B.
  • Entrez Gene IDs:
    112755 (Human); 56216 (Mouse); 24923 (Rat)
  • Alternate Names:
    FP17469; STX1B; STX1B1; STX1B2; Syntaxin 1B; syntaxin 1B1; syntaxin 1B2; syntaxin-1B; syntaxin-1B1; syntaxin-1B2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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