Detects human Syntaxin 1B in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with
recombinant human (rh) Syntaxin 1A and 1B1 is observed, and no cross-reactivity with
rhSyntaxin 5, 6, 7, 8, 12, or 16 is observed.
Monoclonal Mouse IgG1 Clone # 676204
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Syntaxin 1B Lys2-Lys264 Accession # P61266
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat Syntaxin 1B by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, CCD‑1070Sk human foreskin fibroblast cell line, Neuro‑2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Syntaxin 1B Monoclonal Antibody (Catalog # MAB6848) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Syntaxin 1B at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syntaxin 1B
Syntaxin 1B/STX1B is an approximately 33 kDa member of the
Syntaxin family. It is a single-pass type IV
transmembrane protein that is potentially
involved in the docking of synaptic vesicles at presynaptic active zones. The cytoplasmic domain of Syntaxin 1B contains a coiled-coil Syntaxin domain (aa 29-180) which is implicated in the docking of synaptic
vesicles with the presynaptic plasma membrane and a t-SNARE coiled coil domain (aa 191-253). Within the cytoplasmic domain, human Syntaxin 1B shares 100% aa sequence identity with mouse and rat Syntaxin 1B.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.