Syntaxin 1B Antibody (676204)

R&D Systems | Catalog # MAB6848

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 676204
View all Syntaxin 1B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Syntaxin 1B
Lys2-Lys264
Accession # P61266

Specificity

Detects human Syntaxin 1B in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Syntaxin 1A and 1B1 is observed, and no cross-reactivity with rhSyntaxin 5, 6, 7, 8, 12, or 16 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Syntaxin 1B Antibody (676204)

Detection of Human, Mouse, and Rat Syntaxin 1B antibody by Western Blot.

Detection of Human, Mouse, and Rat Syntaxin 1B by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CCD-1070Sk human foreskin fibroblast cell line, Neuro-2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Syntaxin 1B Monoclonal Antibody (Catalog # MAB6848) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Syntaxin 1B at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Syntaxin 1B Antibody (676204)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, CCD‑1070Sk human foreskin fibroblast cell line, Neuro‑2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syntaxin 1B

Syntaxin 1B/STX1B is an approximately 33 kDa member of the Syntaxin family.  It is a single-pass type IV transmembrane protein that is potentially involved in the docking of synaptic vesicles at presynaptic active zones. The cytoplasmic domain of Syntaxin 1B contains a coiled-coil Syntaxin domain (aa 29-180) which is implicated in the docking of synaptic vesicles with the presynaptic plasma membrane and a t-SNARE coiled coil domain (aa 191-253). Within the cytoplasmic domain, human Syntaxin 1B shares 100% aa sequence identity with mouse and rat Syntaxin 1B.

Alternate Names

STX1B, STX1B1, STX1B2

Entrez Gene IDs

112755 (Human); 56216 (Mouse); 24923 (Rat)

Gene Symbol

STX1B

UniProt

P61266

Additional Syntaxin 1B Products

Product Documents for Syntaxin 1B Antibody (676204)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Syntaxin 1B Antibody (676204)

For research use only

Related Research Areas

Neuronal Markers

Citations for Syntaxin 1B Antibody (676204)

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