Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Syntaxin 8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Syntaxin 8
Met1-Gly215
Accession # Q9UNK0

Specificity

Detects human, mouse, and rat Syntaxin 8 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Syntaxin 8 Antibody

Detection of Human, Mouse, and Rat Syntaxin 8 antibody by Western Blot.

Detection of Human, Mouse, and Rat Syntaxin 8 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5448) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Syntaxin 8 was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Syntaxin 8 antibody by Western Blot.

Detection of Human Syntaxin 8 by Western Blot.

Western blot shows recombinant human Syntaxin 12, 16, 1A, 1B2, 5, 6, 7, 8, and 1B1 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5448) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Syntaxin 8 was detected as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Syntaxin 8 antibody in Human Brain by Immunohistochemistry (IHC-P).

Syntaxin 8 in Human Brain.

Syntaxin 8 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human/Mouse/Rat Syntaxin 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5448) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse/Rat Syntaxin 8 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 3 using AF5448 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syntaxin 8

Syntaxin 8 (STX8) is a 28 kDa member of the syntaxin family of proteins. It is ubiquitously expressed, embedded in Golgi, endosomal and lysosomal membranes, and serves as a component of the SNARE complex. STX8 is involved in endosomal homotypic fusion events as well as protein trafficking. Human STX8 is a type IV single-pass transmembrane protein (very short exoplasmic C-terminus) that is 236 amino acids (aa) in length. It contains a coiled-coil region (aa 42‑65), a t-SNARE domain (aa 152‑207) that is likely involved in protein-protein interactions, and a short, four amino acid C-terminal luminal sequence. Over aa 1‑215, human STX8 shares 92% aa identity with mouse STX8.

Alternate Names

CARB, STX8

Entrez Gene IDs

9482 (Human); 55943 (Mouse); 59074 (Rat)

Gene Symbol

STX8

UniProt

Q9UNK0

Additional Syntaxin 8 Products

Product Documents for Human/Mouse/Rat Syntaxin 8 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Syntaxin 8 Antibody

For research use only

Citations for Human/Mouse/Rat Syntaxin 8 Antibody

Customer Reviews for Human/Mouse/Rat Syntaxin 8 Antibody (1)

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  • Human/Mouse/Rat Syntaxin 8 Antibody
    Name: Scott Messenger
    Application: Western Blot
    Sample Tested: T84 human colon carcinoma cell line
    Species: Human
    Verified Customer | Posted 04/25/2017
    100 ug whole cell lysate, 15% gel additional band at ~40kDa
    Human/Mouse/Rat Syntaxin 8 Antibody AF5448

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Protocols

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