Detection of Human/Mouse/Rat Thioredoxin-2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, CH‑1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Thioredoxin‑2 Monoclonal Antibody (Catalog # MAB5765) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin‑2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Rat Thioredoxin‑2 by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Thioredoxin‑2 at approximately 11 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Thioredoxin‑2 Monoclonal Antibody (Catalog # MAB5765). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Thioredoxin-2 (Trx-2; also known as mitochondrial Thioredoxin) belongs to the evolutionarily conserved Thioredoxin family of proteins. Thioredoxins share the Thioredoxin fold containing the active site-CGPC motif. In their reduced form, the active site cysteine residues reduce protein disulfides. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by a NADPH-dependent Thioredoxin reductase. Thioredoxin-2 contains an N-terminal 59 amino acid (aa) transit sequence that is cleaved upon translocation to mitochondria. The amino acid sequence of mature human Thioredoxin-2 is 98% identical to mouse and rat Thioredoxin-2. Thioredoxin-2 interacts with specific components of the mitochondrial respiratory chain and helps regulate the membrane potential. Thioredoxin-2 is ubiquitously but variably expressed and high expression confers resistance to oxidant-induced apoptosis.
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