Human/Mouse/Rat Thioredoxin-2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/Rat Thioredoxin-2 by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human/Mouse/Rat Thioredoxin-2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse and rat Thioredoxin-2 in Western blots.
Monoclonal Mouse IgG1 Clone # 543317
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Thioredoxin-2
Accession # Q99757
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Thioredoxin-2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Thioredoxin-2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, CH-1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Thioredoxin-2 Monoclonal Antibody (Catalog # MAB5765) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human and Rat Thioredoxin-2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Rat Thioredoxin‑2 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Thioredoxin‑2 at approximately 11 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Thioredoxin‑2 Monoclonal Antibody (Catalog # MAB5765). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thioredoxin-2

Thioredoxin-2 (Trx-2; also known as mitochondrial Thioredoxin) belongs to the evolutionarily conserved Thioredoxin family of proteins. Thioredoxins share the Thioredoxin fold containing the active site-CGPC motif. In their reduced form, the active site cysteine residues reduce protein disulfides. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by a NADPH-dependent Thioredoxin reductase. Thioredoxin-2 contains an N-terminal 59 amino acid (aa) transit sequence that is cleaved upon translocation to mitochondria. The amino acid sequence of mature human Thioredoxin-2 is 98% identical to mouse and rat Thioredoxin-2. Thioredoxin-2 interacts with specific components of the mitochondrial respiratory chain and helps regulate the membrane potential. Thioredoxin-2 is ubiquitously but variably expressed and high expression confers resistance to oxidant-induced apoptosis.

Entrez Gene IDs
25828 (Human); 56551 (Mouse); 79462 (Rat)
Alternate Names
mitochondrial thioredoxin; MTRX; MT-TRX; thioredoxin 2; thioredoxin, mitochondrial; Thioredoxin2; Thioredoxin-2; Trx2; TXN2

Product Datasheets

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Citation for Human/Mouse/Rat Thioredoxin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Txn2 haplodeficiency does not affect cochlear antioxidant defenses or accelerate the progression of cochlear cell loss or hearing loss across the lifespan
    Authors: MJ Kim, C Han, K White, HJ Park, D Ding, K Boyd, C Rothenberg, U Bose, P Carmichael, PJ Linser, M Tanokura, R Salvi, S Someya
    Exp. Gerontol., 2020;141(0):111078.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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