Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antibody

(5 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Vang-like Protein 2/VANGL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VANGL1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Vang-like Protein 2/VANGL2
    Gln241-Val521
    Accession # Q9ULK5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat Vang-like Protein 2/
VANGL2 by Western Blot.
Western blot shows lysates of mouse kidney, human and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4815) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vang-like Protein 2/VANGL2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
Vang-like Protein 2/VANGL2 in Mouse Embryo. Vang-like Protein 2/VANGL2 was detected in immersion fixed frozen sections of mouse embryo (E15.5) using Sheep Anti-Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4815) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vang-like Protein 2/VANGL2

VANGL2 (van Gogh-like protein 2; also Strabismus and Ltap) is a 65 kDa member of the Vang family of proteins. It is found in the membranes of embryonic neurons and epithelium where it associates with DVL1-3 and various frizzleds. VANGL2 mutations are associated with neural tube defects. Human VANGL2 is a 521 amino acid (aa) 4-transmembrane protein that contains an N-terminal (aa 1‑108) and C-terminal (aa 239‑521) cytoplasmic domain. There is a Ser-rich region in the N-terminus (aa 5‑20) and a PDZ-binding motif over aa 506‑521. Over aa 241‑521, human, mouse and rat show total identity in amino acid sequence.

  • Entrez Gene IDs:
    57216 (Human); 93840 (Mouse); 289229 (Rat)
  • Alternate Names:
    KIAA1215Loop-tail protein 1 homolog; loop-tail-associated protein; LPP1; LTAP; LTAPVan Gogh-like protein 2; MGC119403; MGC119404; STB1; STB1Strabismus 1; STBM; STBM1; Strabismus; vang (van gogh, Drosophila)-like 2, vang, van gogh-like 2 (Drosophila); Vang like Protein 2; VANGL2; vang-like 2 (van gogh, Drosophila); Vang-like Protein 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Cilia distribution and polarity in the epithelial lining of the mouse middle ear cavity
    Authors: W Luo, H Yi, J Taylor, JD Li, F Chi, NW Todd, X Lin, D Ren, P Chen
    Sci Rep, 2017;7(0):45870.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Planar cell polarity gene expression correlates with tumor cell viability and prognostic outcome in neuroblastoma
    Authors: C Dyberg, P Papachrist, BH Haug, H Lagercrant, P Kogner, T Ringstedt, M Wickström, JI Johnsen
    BMC Cancer, 2016;16(1):259.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. VANGL2 regulates membrane trafficking of MMP14 to control cell polarity and migration.
    J. Cell. Sci., 2012;125(0):2141-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Nodal dependent differential localisation of dishevelled-2 demarcates regions of differing cell behaviour in the visceral endoderm.
    Authors: Trichas G, Joyce B, Crompton LA, Wilkins V, Clements M, Tada M, Rodriguez TA, Srinivas S
    PLoS Biol., 2011;9(2):e1001019.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh/Frozen
  5. Vang-like protein 2 and Rac1 interact to regulate adherens junctions.
    Authors: Lindqvist M, Horn Z, Bryja V, Schulte G, Papachristou P, Ajima R, Dyberg C, Arenas E, Yamaguchi TP, Lagercrantz H, Ringstedt T
    J. Cell. Sci., 2010;123(0):472-83.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
Cell and Tissue Staining Kits
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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
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Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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