Human/Mouse RND3 (RD1 Peptide) Antibody

(1 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human RND3 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 687202
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    RND3 (RD1 peptide)
    Cys197-Arg207
    Accession # P61587
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human RND3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, MCF‑7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human RND3 (RD1 Peptide) Monoclonal Antibody (Catalog # MAB6618) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RND3 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RND3
RND3, also known as RhoE, is an approximately 27 kDa member of the Rho family, small GTPase superfamily of molecules.  It is 244 amino acids (aa) in length, and the mature chain extends from aa 1-241.  RND3 is ubiquitously expressed and is involved in keratinocyte differentiation and stratification.  RND3 overexpression results in cell size enlargement and an increase in the number of stratified cells.  RND3 depletion results in hyperproliferation and delays initiation of keratinocyte differentiation.  Human RND3 shares 100% aa sequence identity with mouse RND3.
  • Long Name:
    Rho family GTPase 3
  • Entrez Gene IDs:
    390 (Human)
  • Alternate Names:
    ARHE; MemB; Protein MemB; Rho family GTPase 3member E; Rho8; RHOE; Rho-related GTP-binding protein Rho8; rho-related GTP-binding protein RhoE; RND3; small GTP binding protein Rho8
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. TGF-beta1 stimulates human Tenon's capsule fibroblast proliferation by miR-200b and its targeting of p27/kip1 and RND3.
    Authors: Tong J, Fu Y, Xu X, Fan S, Sun H, Liang Y, Xu K, Yuan Z, Ge Y
    Invest Ophthalmol Vis Sci, 2014;55(4):2747-56.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human/Mouse RND3 (RD1 Peptide) Antibody MAB6618
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Very Good
 WB Human Anonymous 07/07/2017
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Western Blot Human/Mouse RND3 (RD1 Peptide) Antibody MAB6618
Western Blot: Human/Mouse RND3 (RD1 Peptide) Antibody [MAB6618]

Summary

ApplicationWestern Blot
Sample TestedHEK293 human embryonic kidney cell line
SpeciesHuman

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