Human/Mouse Semaphorin 3C Antibody

Catalog # Availability Size / Price Qty
MAB1728
MAB1728-SP
Semaphorin 3C in MCF‑7 Human Cell Line.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse Semaphorin 3C Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Semaphorin 3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 6B, 6C, 6D, recombinant mouse Semaphorin 3A, 3B, 3E, 3F, 6A, or 7A is observed.
Source
Monoclonal Rat IgG2A Clone # 238835
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3C
Gln24-Ser751 (Arg48Ala, Arg52Ala)
Accession # Q62181
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Semaphorin 3C Fc Chimera, Truncated (Catalog # 1728-S3)
Immunohistochemistry
1-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Semaphorin 3C antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

Semaphorin 3C in MCF‑7 Human Cell Line. Semaphorin 3C was detected in immersion fixed MCF-7 human breast cancer cell line using Rat Anti-Mouse Semaphorin 3C Monoclonal Antibody (Catalog # MAB1728) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Semaphorin 3C antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Semaphorin 3C in Mouse Embryo. Semaphorin 3C was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rat Anti-Human/Mouse Semaphorin 3C Monoclonal Antibody (Catalog # MAB1728) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 3C

Semaphorin 3C (Sema3C; previously semaE) is one of six Class 3 secreted semaphorins which share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development, and may be upregulated in tumor progression (1, 2). The 751 amino acid (aa) mouse Sema3C is highly modular. It contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a cysteine knot, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (1-3). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (4). Mouse Sema3C shares at least 95% aa identity with human, rat, cow and dog Sema3C, and 89% and 75% aa identity with chick and zebrafish Sema3C, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors (1, 2). Sema3C signaling is transduced by Plexin-D1 indirectly via neuropilin-1 or neuropilin-2 receptors (5). Sema3C is expressed in all somitic motor neurons, in lung buds and in cardiac neural crest cells during development (1, 5-8). Sema3C activates integrins in certain cells so, in addition to its repulsive activities, it sometimes acts as a chemoattractant (6, 9). In the developing nervous system, this chemoattraction appears to complement Sema3A repulsion in adjacent cell layers (1, 6, 7). Sema3C also provides an attractive force opposing Sema6A and Sema6B to guide migration of neural crest endothelial cells to the cardiac outflow tract (10). Consequently, defects in aortic arch formation occur when Sema3C or Plexin-D1 genes or Sema3C-neuropilin interactions are disrupted (5, 11, 12).

References
  1. Hinck, L. (2004) Dev. Cell 7:783.
  2. Neufeld, G. et al. (2005) Front. Biosci. 10:751.
  3. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  4. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  5. Gitler, A. D. et al. (2004) Dev. Cell 7:107.
  6. Bagnard, D. et al. (1998) Development 125:5043.
  7. Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767.
  8. Puschel, A. W. et al. (1995) Neuron 14:941.
  9. Herman, J. G. and G. G. Meadows (2007) Int. J. Oncol. 30:1231.
  10. Toyofuku, T. et al. (2008) Dev. Biol. 321:251.
  11. Feiner, L. et al. (2001) Development 128:3061.
  12. Gu, C. et al. (2003) Dev. Cell 5:45.
Entrez Gene IDs
10512 (Human); 20348 (Mouse)
Alternate Names
(semaphorin) 3C; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema E; SEMA3C; SEMAE; Semaphorin 3C; Semaphorin E; semaphorin-3C; semaphorin-E; SEME

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Citation for Human/Mouse Semaphorin 3C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The cleavage of semaphorin 3C induced by ADAMTS1 promotes cell migration.
    Authors: Esselens C, Malapeira J, Colome N, Casal C, Rodriguez-Manzaneque JC, Canals F, Arribas J
    J. Biol. Chem., 2010;285(4):2463-73.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

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Reviews for Human/Mouse Semaphorin 3C Antibody

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Human/Mouse Semaphorin 3C Antibody
By Anonymous on 12/20/2021
Application: IHC Sample Tested: Lung tissue Species: Mouse

Human/Mouse Semaphorin 3C Antibody
By Anonymous on 12/16/2020
Application: ICC/IF Sample Tested: E10.5 mouse embryo fixed in 4% PFA Species: Mouse

Dilution used - 1:200. The staining was done on an E10.5 mouse transverse section (fixed on 4% PFA overnight) and done using standard IF techniques.
The staining was very good on the target region (look at the upper region of picture which shows excellent Semaphorin3C staining on supposed neural crest cells).
However, there's still a little non specific staining on the arterial lining of the heart, which is a small cause of concern.
Probably this could be eliminated by tweaking the fixation/blocking steps.