Human/Mouse SR140 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse SR140 by Western Blot.
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Product Details
Supplemental Products

Human/Mouse SR140 Antibody Summary

Species Reactivity
Human, Mouse
Detects human SR140 in direct ELISAs. Detects human and mouse SR140 in Western blots.
Monoclonal Mouse IgG1 Clone # 983615
Protein A or G purified from hybridoma culture supernatant
Synthetic peptide containing human SR140 Peptide
Accession # O15042
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse SR140 antibody by Western Blot. View Larger

Detection of Human and Mouse SR140 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse SR140 Monoclonal Antibody (Catalog # MAB9897) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SR140 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SR140

SR140, also known as U2-associated protein SR140 (U2SURP) is a 118 kDa protein located in the nucleus. SR140 belongs to the splicing factor SR family and has 3 known isoforms that are produced by alternative splicing. The protein amino acid sequence is highly conserved across species and can be heavily phosphorylated, leading to a shift in observed molecular weight.

Long Name
U2-Associated Protein SR140
Entrez Gene IDs
23350 (Human); 67958 (Mouse); 315903 (Rat)
Alternate Names
140 kDa Ser/Arg-rich domain protein; FSAPa; KIAA0332; SR140; U2 snRNP-associated SURP domain containing; U2 snRNP-associated SURP motif-containing protein; U2-associated protein SR140; U2-Associated SR140 Protein; U2SURP

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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