Human/Mouse SR140 Antibody Summary
Accession # O15042
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse SR140 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse SR140 Monoclonal Antibody (Catalog # MAB9897) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SR140 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SR140, also known as U2-associated protein SR140 (U2SURP) is a 118 kDa protein located in the nucleus. SR140 belongs to the splicing factor SR family and has 3 known isoforms that are produced by alternative splicing. The protein amino acid sequence is highly conserved across species and can be heavily phosphorylated, leading to a shift in observed molecular weight.
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