Human/Mouse Sulfatase-2/SULF2 Antibody Summary
Accession # NP_940998
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Sulfatase‑2/SULF2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Sulfatase-2/SULF2 Monoclonal Antibody (Catalog # MAB7087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Sulfatase-2/SULF2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Sulfatase 2 (SULF2) is a 132 kDa calcium-binding glycoprotein that is one of two extracellular sulfatases (SULFs). SULFs degrade cell surface and extracellular matrix heparin sulfate proteoglycans, altering the binding and signaling of factors such as Wnts and FGFs. Dysregulation of SULF2 is implicated in carcinogenesis. A high molecular weight form (250 kDa) is presumed to be a covalent dimer, while smaller forms (60 kDa and 64 kDa) are produced by proteolytic cleavage. A splicing form, isoform b, differs only in non-coding regions. Within the region used as an immunogen, human SULF2 shares approximately 94% amino acid sequence identity with mouse and rat SULF2, while mouse and rat SULF2 share 98% amino acid sequence identity with each other.
Citation for Human/Mouse Sulfatase-2/SULF2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment
Authors: P Zizza, R Dinami, M Porru, C Cingolani, E Salvati, A Rizzo, C D'Angelo, E Petti, CA Amoreo, M Mottolese, I Sperduti, A Chambery, R Russo, P Ostano, G Chiorino, G Blandino, A Sacconi, J Cherfils-V, C Leonetti, E Gilson, A Biroccio
Nucleic Acids Res., 2019;0(0):.
Sample Types: Whole Cells
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