Detection of Human Sulfatase‑2/SULF2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Sulfatase‑2/SULF2 Monoclonal Antibody (Catalog # MAB7087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Sulfatase‑2/SULF2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Sulfatase 2 (SULF2) is a 132 kDa calcium-binding
glycoprotein that is one of two extracellular sulfatases (SULFs). SULFs degrade
cell surface and extracellular matrix heparin sulfate proteoglycans, altering
the binding and signaling of factors such as Wnts and FGFs. Dysregulation of
SULF2 is implicated in carcinogenesis. A high molecular weight form (250 kDa)
is presumed to be a covalent dimer, while smaller forms (60 kDa and 64 kDa) are
produced by proteolytic cleavage. A splicing form, isoform b, differs only in non-coding regions. Within the region used as an immunogen, human
SULF2 shares approximately 94% amino acid sequence identity with mouse and rat
SULF2, while mouse and rat SULF2 share 98% amino acid sequence identity with each other.
Lemjabbar-Alaoui, H. et al. (2010) Oncogene 29:635.
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