Sulfatase-2/SULF2 Antibody (2B4)

R&D Systems | Catalog # MAB7087

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B4
View all Sulfatase-2/SULF2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Sulfatase-2/SULF2, used to immunize a SULF2-deficient mouse
Accession # NP_940998

Specificity

Detects human Sulfatase-2/SULF2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Sulfatase-2/SULF2 Antibody (2B4)

Detection of Human Sulfatase-2/SULF2 antibody by Western Blot.

Detection of Human Sulfatase‑2/SULF2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Sulfatase-2/SULF2 Monoclonal Antibody (Catalog # MAB7087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Sulfatase-2/SULF2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Sulfatase-2/SULF2 Antibody (2B4)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sulfatase-2/SULF2

Sulfatase 2 (SULF2) is a 132 kDa calcium-binding glycoprotein that is one of two extracellular sulfatases (SULFs). SULFs degrade cell surface and extracellular matrix heparin sulfate proteoglycans, altering the binding and signaling of factors such as Wnts and FGFs. Dysregulation of SULF2 is implicated in carcinogenesis. A high molecular weight form (250 kDa) is presumed to be a covalent dimer, while smaller forms (60 kDa and 64 kDa) are produced by proteolytic cleavage. A splicing form, isoform b, differs only in non-coding regions. Within the region used as an immunogen, human SULF2 shares approximately 94% amino acid sequence identity with mouse and rat SULF2, while mouse and rat SULF2 share 98% amino acid sequence identity with each other.

References

  1. Lemjabbar-Alaoui, H. et al. (2010) Oncogene 29:635.

Alternate Names

SULF2, Sulfatase2

Entrez Gene IDs

55959 (Human); 72043 (Mouse); 311642 (Rat)

Gene Symbol

SULF2

UniProt

NP_940998

Additional Sulfatase-2/SULF2 Products

Product Documents for Sulfatase-2/SULF2 Antibody (2B4)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Sulfatase-2/SULF2 Antibody (2B4)

For research use only

Citations for Sulfatase-2/SULF2 Antibody (2B4)

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