Human/Mouse TGF-beta RI/ALK-5 Antibody

Name: Human/Mouse TGF-beta RI/ALK-5 Antibody
Brand: R&D Systems
SKU: MAB5871-SP
Rating: 3.7 (3 reviews)

Catalog #: MAB5871
6 Citations 3 Reviews Datasheet / COA / SDS

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MAB5871
MAB5871-SP

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Citations (6)

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Reviews (3)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse TGF-beta RI/ALK-5 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse TGF-beta RI/ALK-5 in Western blots. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with rrMIS RII, rhTGF‑ beta RII, rhTGF‑ beta RIIb, or rhTGF‑ beta RIII.

Source

Monoclonal Rat IgG_2A Clone # 141231

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta RI/ALK-5
Ala21-Glu121
Accession # BAA05023

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse TGF-beta RI/ALK‑5 Fc Chimera (Catalog # 587-RI) and Recombinant Human TGF-beta RI/ALK‑5 Fc Chimera (Catalog # 3025-BR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RI/ALK-5

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor‑like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

References

Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.

Long Name

Transforming Growth Factor beta Receptor I

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Alternate Names

AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 2.7.11.30; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse TGF-beta RI/ALK-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Prevention of bleomycin-induced pulmonary fibrosis by a RANKL peptide in mice
Authors: N Ju, H Hayashi, M Shimamura, S Baba, S Yoshida, R Morishita, H Rakugi, H Nakagami
Scientific Reports, 2022;12(1):12474.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
Proc Natl Acad Sci U S A, 2015;112(9):E957-65.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Beclin 1 regulates neuronal transforming growth factor-beta signaling by mediating recycling of the type I receptor ALK5.
Authors: O'Brien C, Bonanno L, Zhang H, Wyss-Coray T
Mol Neurodegener, 2015;10(1):69.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-Fr
Integrin alpha3beta1 potentiates TGFbeta-mediated induction of MMP-9 in immortalized keratinocytes.
Authors: Lamar JM, Iyer V, DiPersio CM
J. Invest. Dermatol., 2007;128(3):575-86.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis.
Authors: Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J
Nat Commun, 0;7(0):11012.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
Fibulin-6 regulates pro-fibrotic TGF-beta responses in neonatal mouse ventricular cardiac fibroblasts.
Authors: Chowdhury A, Hasselbach L, Echtermeyer F, Jyotsana N, Theilmeier G, Herzog C
Sci Rep, 0;7(0):42725.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot