Human/Mouse UNC13D Antibody

Recombinant Monoclonal Antibody..
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse UNC13D in Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1224B
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    KLH-coupled N-terminal human UNC13D peptide
    Accession # Q70J99
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse UNC13D by Western Blot. Western blot shows lysates of NK‑92 human natural killer lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, Daudi human Burkitt's lymphoma cell line, CH‑1 mouse B cell lymphoma cell line, and CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse UNC13D Monoclonal Antibody (Catalog # MAB89662) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for UNC13D at approximately 95-115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse UNC13D by Simple WesternTM. Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, CH‑1 mouse B cell lymphoma cell line, and CTLL‑2 mouse cytotoxic T cell line, loaded at 0.2 mg/mL. A specific band was detected for UNC13D at approximately 112-122 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse UNC13D Monoclonal Antibody (Catalog # MAB89662). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: UNC13D
UNC13D (also Munc13-4) is a 123 kDa cytoplasmic and peripheral membrane protein that is expressed at highest levels in hematopoietic tissues.  UNC13D appears to play a role in vesicle maturation during exocytosis and its expression is obligatory for exocytosis of cytolytic granules from NK and T cells. A point mutation in intron 1 of UNC13D causes familial hematophagocytic lymphohistiocytosis type3, a rare autosomal recessive immune deficiency. The mutation disrupts transcription factor binding and prevents expression of an alternative isoform that is required for lymphocyte cytotoxicity. The conventional and alternative isoforms are identical from amino acids (aa) 40-1090 but have distinct N-terminal segments.  Human and mouse have 75% aa sequence identity throughout the region used as the immunogen.  It is unknown whether mice express the alternative isoform. 
  • Long Name:
    UNC-13 Homolog D
  • Entrez Gene IDs:
    201294 (Human); 70450 (Mouse); 192177 (Rat)
  • Alternate Names:
    FHL3; HLH3; HPLH3; Munc13-4; Munc13-4protein unc-13 homolog D; unc-13 homolog D (C. elegans); UNC13D
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