Detection of Human MRGPRF by Western Blot. Western blot shows lysates of human uterus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MRGPRF Monoclonal Antibody (Catalog # MAB8396) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MRGPRF at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of MRGPRF in HEK293 Human Cell Line Transfected with Human MRGPRF and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human MRGPRF or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human MRGPRF Monoclonal Antibody (Catalog # MAB8396) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MRGPRF (MAS-related GPR, Member F), also known as RTA, MRGF, GPR140 or GPR168, belongs to the G protein coupled receptor 1 family. MRGPRF may be upregulated during the process of CSF-1 or GM-CSF-induced human monocyte to macrophage differentiation (1).
Hohenhaus D.M., et al. (2013) Immunobiology 218:1345.
Mas-related G Protein-coupled Receptor Member F
Entrez Gene IDs:
116535 (Human); 211577 (Mouse); 266762 (Rat)
FLJ16111; FLJ29034; FLJ40998; FLJ53714; G protein-coupled receptor 140; G protein-coupled receptor 168; G protein-coupled receptor MrgF; GPR140; GPR168; G-protein coupled receptor 140; G-protein coupled receptor 168; mas-related G protein-coupled MRGF; Mas-related gene F protein; MAS-related GPR, member F; mas-related G-protein coupled receptor member F; MGC21621; mrgF; MRGFDKFZp586B2122; RTA; seven transmembrane helix receptor
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