Detection of NAALADase‑like 2/ NAALADL2 in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human NAALADase‑like 2/NAALADL2 Monoclonal Antibody (Catalog # MAB4665, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NAALADase-like 2/NAALADL2
NAALADL2 (N-acetylated alpha -linked acidic dipeptidase like 2) is a member
of the peptidase M28 family of enzymes. It is presumably a type II
transmembrane (TM) protein that may have O-glycosyl hydrolase activity.
Human NAALADL2 is 795 amino acids (aa) in length. It is believed to
contain a cytoplasmic and TM segment at the N-terminus, followed by a
peptidase domain (aa 444‑596) and a TfR-like dimerization region (aa
688‑777). There are multiple splice forms. One shows a 4 aa substitution
for aa 292‑795, a second shows an alternate start site at Met18 with a
24 aa substitution for aa 314‑795, and a third contains an alternate
start site at Met283, accompanied by a deletion of aa 363‑411 and
633‑795. Over aa 152‑795, human NAALADL2 shares 87% and 82% aa sequence
identity with mouse and canine NAALADL2, respectively.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.