Human Neprilysin-2/MMEL1 Antibody

Catalog # Availability Size / Price Qty
MAB2340
MAB2340-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human Neprilysin-2/MMEL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neprilysin‑2/MMEL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Neprilysin, rhECE‑1 or rhECE‑2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 353207
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neprilysin-2
Gly78-Trp779
Accession # Q495T6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neprilysin-2/MMEL1

Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles.

References
  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Carpentier, M. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 426.
Entrez Gene IDs
79258 (Human)
Alternate Names
EC 3.4.24; EC 3.4.24.11; MELL1; membrane metallo-endopeptidase-like 1; Membrane metallo-endopeptidase-like 2MGC119455; MGC119454; MMEL1; MMEL2; NEP2; NEP2(m); NEPIIMGC119456; Neprilysin II; Neprilysin2; Neprilysin-2; NL2; NL2NL1; SEP; soluble secreted endopeptidase; zinc metallopeptidase

Product Datasheets

You must select a language.

x

Citation for Human Neprilysin-2/MMEL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human membrane metallo-endopeptidase-like protein degrades both beta-amyloid 42 and beta-amyloid 40.
    Authors: Huang JY, Bruno AM, Patel CA, Huynh AM, Philibert KD, Glucksman MJ, Marr RA
    Neuroscience, 2008-05-16;155(1):258-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Neprilysin-2/MMEL1 Antibody

There are currently no reviews for this product. Be the first to review Human Neprilysin-2/MMEL1 Antibody and earn rewards!

Have you used Human Neprilysin-2/MMEL1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review