Human Nesfatin-1/Nucleobindin-2 Antibody

R&D Systems | Catalog # AF5949

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Nesfatin-1/Nucleobindin-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Nesfatin‑1/Nucleobindin‑2
Pro26-Leu106
Accession # P80303

Specificity

Detects human Nesfatin‑1/Nucleobindin‑2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Nesfatin-1/Nucleobindin-2 Antibody

Detection of Human Nesfatin-1/Nucleobindin-2 antibody by Western Blot.

Detection of Human Nesfatin‑1/Nucleobindin‑2 by Western Blot.

Western blot shows lysates of TK-6 human lymphoblast cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Nesfatin-1/Nucleobindin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5949) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Nesfatin-1/ Nucleobindin-2 between 10 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Nesfatin-1/Nucleobindin-2 antibody in Human Brain by Immunohistochemistry (IHC-P).

Nesfatin‑1/Nucleobindin‑2 in Human Brain.

Nesfatin-1/Nucleobindin-2 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human Nesfatin-1/Nucleobindin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5949) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Nesfatin-1/Nucleobindin-2 antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC).

Nesfatin‑1/Nucleobindin‑2 in Human Mesenchymal Stem Cells.

Nesfatin-1/Nucleobindin-2 was detected in immersion fixed human mesenchymal stem cells differentiated into adipocytes using Sheep Anti-Human Nesfatin-1/Nucleobindin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5949) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human Nesfatin-1/Nucleobindin-2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated into adipocytes

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain  (hypothalamus)

Western Blot

1 µg/mL
Sample: TK‑6 human lymphoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nesfatin-1/Nucleobindin-2

Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1-24), Nesfatin-1 (aa 25-106), a DNA-binding site (aa 171-223), and two EF-hand regions (aa 241-276 and 293-328). Intracellular uncleaved NUCB2 is 45-50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109-187) and Nesfatin-3 (aa 190-420). Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.

Alternate Names

NEFA, Nesfatin1, NUCB2, Nucleobindin-2

Entrez Gene IDs

4925 (Human); 53322 (Mouse); 59295 (Rat)

Gene Symbol

NUCB2

UniProt

P80303 (Human)

Additional Nesfatin-1/Nucleobindin-2 Products

Product Documents for Human Nesfatin-1/Nucleobindin-2 Antibody

Certificate of Analysis

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Product Specific Notices for Human Nesfatin-1/Nucleobindin-2 Antibody

For research use only

Citations for Human Nesfatin-1/Nucleobindin-2 Antibody

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Protocols

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