Human NETO1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human NETO1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human NETO2 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human NETO1
    Thr23-Val345
    Accession # Q8TDF5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human NETO1
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
NETO1 in MOLT‑4 Human Cell Line. NETO1 was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Sheep Anti-Human NETO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4337) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
NETO1 in Human Prostate. NETO1 was detected in immersion fixed paraffin-embedded sections of human prostate using Sheep Anti-Human NETO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4337) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NETO1

Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina. The 511 amino acid residue mature human NETO1 contains an extracellular domain with two CUB domains (aa 41‑155 and 172‑287), and one class A LDL-receptor segment (aa 292‑326). Three human NETO1 splice isoforms exist, including a secreted soluble variant that shows an Asp-to-Glu transition at residue 157 followed by a premature truncation. Over aa 23‑345, human NETO1 shares 97% and 98% aa identity with mouse and canine NETO1, respectively.

  • Long Name:
    Neuropilin [NRP] And Tolloid [TLL]-like 1
  • Entrez Gene IDs:
    81832 (Human); 246317 (Mouse); 307206 (Rat)
  • Alternate Names:
    BCTL1; Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 1; BTCL1; BTCL1BCTL1neuropilin and tolloid-like protein 1; FLJ41325; NETO1; neuropilin (NRP) and tolloid (TLL)-like 1
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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