Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Neuronal Pentraxin R/NPTXR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Pentraxin R/NPTXR
Ala24-Lys499
Accession # O95502

Specificity

Detects human Neuronal Pentraxin R/NPTXR in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) NPTX2 and rhNPTX1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Neuronal Pentraxin R/NPTXR Antibody

Detection of Human Neuronal Pentraxin R/NPTXR antibody by Simple WesternTM.

Detection of Human Neuronal Pentraxin R/NPTXR by Simple WesternTM.

Simple Western lane view shows lysates of U-87 MG human glioblastoma/astrocytoma cell line and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for Neuronal Pentraxin R/NPTXR at approximately 66 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Neuronal Pentraxin R/NPTXR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4414) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Neuronal Pentraxin R/NPTXR in Hippocampus.

Neuronal Pentraxin R/NPTXR was detected in immersion fixed paraffin-embedded sections of Hippocampus using Sheep Anti-Human Neuronal Pentraxin R/NPTXR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4414) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Neuronal Pentraxin R/NPTXR by Western Blot

Detection of Neuronal Pentraxin R/NPTXR by Western Blot

NPTX levels are reduced in CSF from individuals with clinically diagnosed AD.(A,B) Western blot assays of NPTX2, NPTX1 and NPTXR in lumbar cerebrospinal fluid (CSF) from age-matched controls and patients with clinically diagnosed AD. AD patients show reduced NPTX2, NPTX1 and NPTXR levels in CSF. Control, n = 36; AD, n = 30. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28440221), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Neuronal Pentraxin R/NPTXR by Western Blot

Detection of Neuronal Pentraxin R/NPTXR by Western Blot

NPTX2 levels are reduced in human postmortem AD brain and DS brain, but not in ASYMAD brain. Representative western blot images (A) of NPTX2, NPTX1, and NPTXR, normalized to PSD95 in the frontopolar cortex (FPC), precuneus (PCU), occipital gyrus (OCC), middle frontal gyrus (MFG), middle temporal gyrus (MTG) and parietal gyrus (PAR) from controls and AD subjects. NPTX2 is down-regulated in all assayed brain regions of AD individuals. FPC: control, n = 7; AD, n = 8. PCU: control, n = 15; AD, n = 19. OCC: control, n = 7; AD, n = 7. MFG: control, n = 9; AD, n = 16. MTG: control, n = 10; AD, n = 18. PAR: control, n = 5; AD, n = 5. (C) Nptx2 mRNA is reduced in AD brain. FPC: control, n = 9; AD, n = 16. PCU: control, n = 7; AD, n = 6. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28440221), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Neuronal Pentraxin R/NPTXR by Western Blot

Detection of Neuronal Pentraxin R/NPTXR by Western Blot

Reduction of NPTXR levels in lumbar cerebrospinal fluid (CSF) from individuals with AD.(A) Representative western blot images show three major bands with human CSF samples by NPTXR antibody. (B) Patients with clinically diagnosed AD have reduced NPTXR levels in CSF compared with healthy controls. Control, n = 31; AD, n = 28. ***p<0.001 by two-tailed t test. Data represent mean ± SEM.DOI:http://dx.doi.org/10.7554/eLife.23798.019 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28440221), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Neuronal Pentraxin R/NPTXR Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Hippocampus

Simple Western

20 µg/mL
Sample: U‑87 MG human glioblastoma/astrocytoma cell line and Human hippocampus tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human Neuronal Pentraxin R/NPTXR

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuronal Pentraxin R/NPTXR

NPTXR (Neuronal Pentraxin Receptor; also NPR) is a 65 kDa, type II transmembrane glycoprotein that binds Neuronal Pentraxins 1 and 2. It is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions. Human NPTXR is 499 amino acids (aa) in length. It contains a two aa cytoplasmic tail, a 21 aa transmembrane segment, and a 476 aa extracellular region with one Pentraxin domain (aa 295‑499). One alternate start site exists at Met12, and a 55 kDa soluble form (possibly beginning at Gln56) may be generated by proteolytic cleavage. Over aa 24‑499, human NPTXR is 87% aa identical to mouse NPTXR.

Alternate Names

NPR, NPTXR

Entrez Gene IDs

23467 (Human); 73340 (Mouse); 81005 (Rat)

Gene Symbol

NPTXR

UniProt

O95502

Additional Neuronal Pentraxin R/NPTXR Products

Product Documents for Human Neuronal Pentraxin R/NPTXR Antibody

Certificate of Analysis

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Product Specific Notices for Human Neuronal Pentraxin R/NPTXR Antibody

For research use only

Related Research Areas

Acute Phase Proteins

Citations for Human Neuronal Pentraxin R/NPTXR Antibody

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Protocols

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