Human NFkB1 Antibody

Catalog # Availability Size / Price Qty
MAB2697
MAB2697-SP
Human NFkB1 Antibody in Data
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Product Details
Citations (1)
FAQs
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Human NFkB1 Antibody Summary

Species Reactivity
Human
Specificity
Detect endogenous human NF kappa B1 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 285412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NF kappa B1
Pro317-Asp437
Accession # P19838
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human NF?B1 antibody by Western Blot. View Larger

Detection of Human NF kappa B1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse NF?B1 Monoclonal Antibody (Catalog # MAB2697) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NF?B1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFkB1

Nuclear Factor kappa B1 (NF kappa B1 or NF kappa B p50) is a member of the NF kappa B/Rel family of transcription factors. NF kappa B1 dimerizes with other members of the NF kappa B/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.

Long Name
Nuclear Factor Kappa-B, Subunit 1
Entrez Gene IDs
4790 (Human); 18033 (Mouse)
Alternate Names
DNA-binding factor KBF1; EBP-1; NF-kappa-B; NFkB1; NF-kB1; NFKB-p105; Nuclear factor NF-kappa-B p105 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p105

Product Datasheets

Citation for Human NFkB1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CD40 promotion of amyloid beta production occurs via the NF-kappaB pathway.
    Authors: Ait-Ghezala G, Volmar CH, Frieling J, Paris D, Tweed M, Bakshi P, Mullan M
    Eur. J. Neurosci., 2007;25(6):1685-95.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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