Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Ninjurin-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Ninjurin-1
Asp2-Val81
Accession # Q92982

Specificity

Detects human Ninjurin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human Ninjurin-2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Ninjurin‑1 Antibody

Detection of Human Ninjurin‑1 antibody by Western Blot.

Detection of Human Ninjurin‑1 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Ninjurin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5105) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ninjurin-1 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Ninjurin-1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Ninjurin‑1 in Human Liver.

Ninjurin-1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Ninjurin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5105) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Ninjurin‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ninjurin-1

Ninjurin-1 (nerve injury-induced protein 1) is a 20-22 kDa member of the Ninjurin family of transmembrane (TM) proteins. It is expressed by Schwann cells, neurons and hepatocytes and participates in intercellular homophilic binding during nerve regeneration. Human Ninjurin-1 is 152 amino acids in length. It has an unusual membrane orientation. There is an 80 amino acid (aa) N-terminal extracellular domain (ECD) (aa 1-80), followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 142-152). Homophilic binding is divalent-cation dependent and occurs between Pro26 and Asn37. Over aa 1-80, human Ninjurin-1 shares 84% aa sequence identity with mouse Ninjurin-1. Human Ninjurin-1 shares only 50% aa sequence identity with human Ninjurin-2.

Alternate Names

NIN1, NINJ1, Ninjurin1

Entrez Gene IDs

4814 (Human); 18081 (Mouse); 25338 (Rat)

Gene Symbol

NINJ1

UniProt

Q92982

Additional Ninjurin-1 Products

Product Documents for Human Ninjurin‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Ninjurin‑1 Antibody

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

Citations for Human Ninjurin‑1 Antibody

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Protocols

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