Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 411913
View all Notch-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Notch‑4 Intracellular Domain
Gly1778-Lys2003
Accession # Q99466

Specificity

Detects human Notch‑4 Intracellular Domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Notch-1 Intracellular Domain, rhNotch-2 Intracellular Domain, rhNotch-3, recombinant rat (rr) Notch-1 EC,rrNotch‑2 EC, or rrDelta-1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Notch‑4 Intracellular Domain Antibody

Notch-4 antibody in Human HUVEC by Immunocytochemistry (ICC).

Notch‑4 in Human HUVEC.

Notch-4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Notch-4 Intracellular Domain Monoclonal Antibody (Catalog # MAB3847) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Notch‑4 Intracellular Domain Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: Recombinant Human Notch‑4 Intracellular Domain

Reviewed Applications

Read 4 reviews rated 4 using MAB3847 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Notch-4

Notch-4 is a 250 kDa member of the NOTCH family of proteins. It is primarily expressed on endothelium. Human Notch-4 is a 1980 amino acid (aa) type I transmembrane (TM) glycoprotein that contains a 1424 aa extracellular domain (ECD) and a 535 aa cytoplasmic region. Notch-4 undergoes proteolytic processing at multiple sites. There is probable cleavage of the ECD by furin in the Golgi between aa 1310‑1410. This creates a mature, potentially disulfide-linked heterodimer at the cell surface. Upon ligand binding, two sequential cleavages occur, one in the ECD after Ala1431, and a second in the TM domain after Lys1466. This creates a soluble cytoplasmic fragment that interacts with RBP-J kappa and activates genes associated with HES-1. Over cytoplasmic aa 1778‑2003, human Notch‑4 is 78% aa identical to mouse Notch-4.

Alternate Names

INT3, Notch4

Entrez Gene IDs

4855 (Human); 18132 (Mouse); 406162 (Rat)

Gene Symbol

NOTCH4

UniProt

Q99466

Additional Notch-4 Products

Product Documents for Human Notch‑4 Intracellular Domain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Notch‑4 Intracellular Domain Antibody

For research use only

Related Research Areas

Notch Pathway

Citations for Human Notch‑4 Intracellular Domain Antibody

Customer Reviews for Human Notch‑4 Intracellular Domain Antibody (4)

4 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
50%
3 Stars
25%
2 Stars
0%
1 Stars
0%

Have you used Human Notch‑4 Intracellular Domain Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Human Notch-4 Intracellular Domain Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 06/28/2022
    Human Notch‑4 Intracellular Domain Antibody MAB3847
  • Human Notch-4 Intracellular Domain Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Notch‑4 Intracellular Domain Antibody MAB3847
  • Human Notch-4 Intracellular Domain Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Notch‑4 Intracellular Domain Antibody MAB3847
  • Human Notch-4 Intracellular Domain Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies