Human Notch-4 Intracellular Domain Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Notch‑4 Intracellular Domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Notch-1 Intracellular Domain, rhNotch-2 Intracellular Domain, rhNotch-3, recombinant rat (rr) Notch-1 EC,
    rrNotch‑2 EC, or rrDelta-1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 411913
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Notch‑4 Intracellular Domain
    Gly1778-Lys2003
    Accession # Q99466
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Notch‑4 Intracellular Domain
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Notch‑4 in Human HUVEC. Notch‑4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Notch‑4 Intracellular Domain Monoclonal Antibody (Catalog # MAB3847) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Notch-4
Notch-4 is a 250 kDa member of the NOTCH family of proteins. It is primarily expressed on endothelium. Human Notch-4 is a 1980 amino acid (aa) type I transmembrane (TM) glycoprotein that contains a 1424 aa extracellular domain (ECD) and a 535 aa cytoplasmic region. Notch-4 undergoes proteolytic processing at multiple sites. There is probable cleavage of the ECD by furin in the Golgi between aa 1310‑1410. This creates a mature, potentially disulfide-linked heterodimer at the cell surface. Upon ligand binding, two sequential cleavages occur, one in the ECD after Ala1431, and a second in the TM domain after Lys1466. This creates a soluble cytoplasmic fragment that interacts with RBP-J kappa and activates genes associated with HES-1. Over cytoplasmic aa 1778‑2003, human Notch‑4 is 78% aa identical to mouse Notch-4.
  • Entrez Gene IDs:
    4855 (Human); 18132 (Mouse); 406162 (Rat)
  • Alternate Names:
    INT3; neurogenic locus notch homolog protein 4; notch 4; Notch homolog 4; NOTCH3; Notch4; Notch-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. KSHV-induced notch components render endothelial and mural cell characteristics and cell survival.
    Authors: Liu R, Li X, Tulpule A, Zhou Y, Scehnet JS, Zhang S, Lee JS, Chaudhary PM, Jung J, Gill PS
    Blood, 2010;115(4):887-95.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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