Detection of Human Notum by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Notum Monoclonal Antibody (Catalog # MAB9356) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Notum at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to
the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions
as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl
moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the
Frizzled-8 receptor (1, 2). It also induces the release of cell surface
GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum
regulates head regeneration in planaria (3), head formation and neural
induction in Xenopus (1), and long range activity of hedgehog proteins in
Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and
colorectal cancers (6, 7). Within amino acids 81-451, human Notum shares 92% aa
sequence identity with mouse and rat Notum.
Zhang, X. et al. (2015) Dev. Cell 32:719.
Kakugawa, S. et al. (2015) Nature 519:187.
Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
Traister, A. et al. (2008) Biochem. J. 410:503.
Ayers, K.L. et al. (2010) Dev. Cell 18:605.
Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
De Robertis, M. et al. (2015) Oncotarget 6:41237.
Entrez Gene IDs:
147111 (Human); 77583 (Mouse); 303743 (Rat)
EC 3.-; notum pectinacetylesterase homolog (Drosophila); Notum; protein notum homolog
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