Key Product Details

Species Reactivity

Validated:

Human

Cited:

Insect

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 377029
View all NPRA/NPR1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NPRA/NPR1
Gly33-Glu473
Accession # P16066

Specificity

Detects human NPRA/NPR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse NPR1 or recombinant human NPR2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human NPRA/NPR1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human NPRA/NPR1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NPRA/NPR1

Natriuretic peptide receptor A/guanylate cyclase A (NPR1), also called NPRA or GC-A, is a 120‑140 kDa type I transmembrane glycoprotein that is the primary receptor for natrietic peptides ANP and BNP. Binding of ANP to the extracellular ligand binding domain (aa 54‑415), plus ATP to the intracellular kinase homology domain (aa 528‑805) activates a cytoplasmic guanylate cyclase (aa 840‑1023). NPR1 is expressed most highly in kidney, adrenal and adipose tissue. Human NPR1 extracellular domain shows 86%, 44% and 34% aa identity with mouse NPR1, human NPRB and human NPRC, respectively.

Long Name

Natriuretic Peptide Receptor A

Alternate Names

ANPa, ANPRA, GC-A, GUCY2A, NPR1, NPRA, Pndr

Entrez Gene IDs

4881 (Human); 18160 (Mouse); 24603 (Rat)

Gene Symbol

NPR1

UniProt

P16066

Additional NPRA/NPR1 Products

Product Documents for Human NPRA/NPR1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NPRA/NPR1 Antibody

For research use only

Citations for Human NPRA/NPR1 Antibody

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