Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 557521
View all NPY2R Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived transfected with recombinant human NPY2R
Met1-Val381
Accession # P49146

Specificity

Detects human NPY2R in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NPY2R Antibody

Detection of NPY2R antibody in HEK293 Human Cell Line Transfected with Human NPY2R and eGFP antibody by Flow Cytometry.

Detection of NPY2R in HEK293 Human Cell Line Transfected with Human NPY2R and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) Human NPY2R or (B) irrelevant protein, and EGFP, was stained with Mouse Anti-Human NPY2R Monoclonal Antibody (Catalog # MAB10211) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 isotype control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human NPY2R Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human NPY2R and eGFP

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NPY2R

NPY2R is a member of the G protein-coupled receptor 1 family. Functionally, NPY2R is a receptor for neuropeptide Y and peptide YY. Neuropeptide Y is one of the most abundant neuropeptides in the mammalian nervous system and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system. NPY receptors represent novel molecular therapeutic targets in cancer and obesity.

Long Name

Neuropeptide Y Receptor Y2

Alternate Names

Y2 Receptor

Entrez Gene IDs

4887 (Human); 18167 (Mouse); 66024 (Rat)

Gene Symbol

NPY2R

UniProt

P49146

Additional NPY2R Products

Product Documents for Human NPY2R Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NPY2R Antibody

For research use only

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