Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1284B
View all OPA1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region within amino acid residues 500-600 of human OPA1
Accession # O60313

Specificity

Detects human, mouse, and rat OPA1 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human OPA1 Antibody

Detection of Human, Mouse, and Rat OPA1 antibody by Western Blot.

Detection of Human, Mouse, and Rat OPA1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PANC-1 human pancreatic carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human OPA1 Monoclonal Antibody (Catalog # MAB9506) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for OPA1 at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human OPA1 by Simple WesternTM.

Simple Western shows lysates of MDA‑MB‑231 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for OPA1 at approximately 90-96 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human OPA1 Monoclonal Antibody (Catalog # MAB9506). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human OPA1 Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: MDA-MB-231 human breast cancer cell line

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, PANC-1 human pancreatic carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OPA1

Optic Atrophy-1 (OPA1), aka Dynamin-like 120 kDa protein, mitochondrial, is a Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. OPA1 exists as a single-pass membrane protein in the mitochondrion inner membrane as well as in soluble forms in mitochondrion intermembrane space, and is expressed in retina, brain, testis, heart, skeletal muscles. Human OPA1 binds PARL and interacts with CHCHD3 as well as IMMT (preferentially with soluble OPA1 forms). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into mitochondrial intermembrane space. OPA1 protein form S1 is an inactive form produced by cleavage at S1 position by metalloendopeptidase OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion. Defects in OPA1 have been linked to optic atrophy type 1 (OPA1) and dominant optic atrophy plus syndrome (DOA+).

Long Name

Optic Atrophy Protein 1

Alternate Names

BERHS, EC 3.6.5.5, LargeG, lilr3, MGM1, MTDPS14, NPG, NTG

Entrez Gene IDs

4976 (Human); 74143 (Mouse); 171116 (Rat)

Gene Symbol

OPA1

UniProt

O60313

Additional OPA1 Products

Product Documents for Human OPA1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human OPA1 Antibody

For research use only

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