Human OPA1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat OPA1 by Western Blot.
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Product Details
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Human OPA1 Antibody Summary

Species Reactivity
Detects human, mouse, and rat OPA1 in Western blots.
Recombinant Monoclonal Rabbit IgG Clone # 1284B
Protein A or G purified from cell culture supernatant
A synthetic peptide made to an internal region within amino acid residues 500-600 of human OPA1
Accession # O60313
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat OPA1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat OPA1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PANC-1 human pancreatic carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human OPA1 Monoclonal Antibody (Catalog # MAB9506) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for OPA1 at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OPA1

Optic Atrophy-1 (OPA1), aka Dynamin-like 120 kDa protein, mitochondrial, is a Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. OPA1 exists as a single-pass membrane protein in the mitochondrion inner membrane as well as in soluble forms in mitochondrion intermembrane space, and is expressed in retina, brain, testis, heart, skeletal muscles. Human OPA1 binds PARL and interacts with CHCHD3 as well as IMMT (preferentially with soluble OPA1 forms). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into mitochondrial intermembrane space. OPA1 protein form S1 is an inactive form produced by cleavage at S1 position by metalloendopeptidase OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion. Defects in OPA1 have been linked to optic atrophy type 1 (OPA1) and dominant optic atrophy plus syndrome (DOA+).

Long Name
Optic Atrophy Protein 1
Entrez Gene IDs
4976 (Human); 74143 (Mouse); 171116 (Rat)
Alternate Names
BERHS; EC; FLJ12460; KIAA0567dynamin-like 120 kDa protein, mitochondrial; LargeG; lilr3; MGM1; mitochondrial dynamin-like GTPase; MTDPS14; NPG; NPGlargeG; NTG; NTGmitochondrial dynamin-like 120 kDa protein; OPA1; optic atrophy 1 (autosomal dominant); Optic atrophy protein 1

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