Human PD-ECGF/Thymidine Phosphorylase Antibody

Catalog # Availability Size / Price Qty
MAB1646
MAB1646-SP
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Human PD-ECGF/Thymidine Phosphorylase Antibody Summary

Species Reactivity
Human
Specificity
Detects human PD-ECGF/Thymidine Phosphorylase in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 27917
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PD-ECGF/Thymidine Phosphorylase
Ala11-Gln482
Accession # P19971
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human PD-ECGF/Thymidine Phosphorylase (Catalog # 229-PE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-ECGF/Thymidine Phosphorylase

PD-ECGF, also known as Thymidine Phosphorylase and Gliostatin, is produced by placenta, platelets, liver, lung, spleen, lymph nodes, and peripheral lymphocytes. It is overexpressed by many tumors in response to chemical or physical stress. PD-ECGF promotes endothelial cell proliferation and chemotaxis and promotes angiogenesis. PD-ECGF is a key enzyme in the pyrimidine nucleoside salvage pathway. It also metabolizes and inactivates chemotherapeutic drugs such as 5‑fluorouracil and its derivatives. The human PD‑ECGF cDNA encodes a 482 amino acid (aa) polypeptide with a 10 aa propeptide. The protein is localized mostly within the producer cells. N-terminal truncation, resulting in proteins lacking 10 aa and 6 aa, has been observed in PD-ECGF purified from platelets and placenta, respectively. In solution, PD-ECGF exists as a non-disulfide linked homodimer.

References
  1. Haraguchi, M. et al. (1994) Nature 368:198.
  2. Toi, M. et al. (2005) Lancet Oncol. 6:158.
  3. Akiyama, S. et al. (2004) Canc. Sci. 95:851.
Long Name
Platelet-derived Endothelial Cell Growth Factor
Entrez Gene IDs
1890 (Human); 72962 (Mouse)
Alternate Names
dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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