Human PDGF Antibody

Catalog # Availability Size / Price Qty
AB-20-NA
Human PDGF Antibody in Neutralization
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Product Details
Citations (3)
FAQs
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Human PDGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) PDGF‑AA, 60% cross-reactivity with rhPDGF-AB, and nearly 100% cross-reactivity with rhPDGF-BB is observed.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
Human platelet-derived PDGF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Human PDGF (Catalog # 120-HD)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize PDGF-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Rizzino, A. et al. (1988) Cancer Res. 48:4266]. The Neutralization Dose (ND50) is typically 5-10 µg/mL in the presence of 10 ng/mL Human PDGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by PDGF and Neutralization by Human PDGF Antibody. View Larger

Cell Proliferation Induced by PDGF and Neutralization by Human PDGF Antibody. Human PDGF (Catalog # 120-HD) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Human PDGF (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human PDGF Polyclonal Antibody (Catalog # AB-20-NA). The ND50is typically 5-10 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF

PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimers are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Although all three isoforms of PDGF exist in human platelets, R&D Systems hPDGF consists predominantly of hPDGF-AB heterodimers.

Two distinct PDGF receptors, the alpha -receptor and the beta -receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha -receptor binds both the A and B chains with high affinity whereas the beta -receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding.

In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells and neutrophils and has been reported to be important in the modification of cellular matrix constituents.

Long Name
Platelet-derived Growth Factor
Entrez Gene IDs
5154 (Human)
Alternate Names
PDGF; PDGF1; PDGF-1; PDGFA

Product Datasheets

Citations for Human PDGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity.
    Authors: Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ
    Brain, 2010;133(0):2232-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
    Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
    Exp. Hematol., 2009;37(7):849-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Temporally expressed PDGF and FGF-2 regulate embryonic coronary artery formation and growth.
    Authors: Tomanek RJ, Hansen HK, Christensen LP
    Arterioscler. Thromb. Vasc. Biol., 2008;28(7):1237-43.
    Species: Avian
    Sample Types: Whole Tissue
    Applications: Neutralization

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