Detection of Human Phospho-ATM (S1981) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-ATM (S1981) Monoclonal Antibody (Catalog # MAB22902) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for phospho-ATM (S1981) at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
ATM (Ataxia Telangiectasia Mutated) is a
350-370 kDa member of the ATM subfamily, PI3/PI4-kinase family of enzymes. It
is ubiquitously expressed and serves as a DNA damage sensor. ATM is activated
via autophosphorylation in response to double strand DNA breaks. Activated ATM
phosphorylates multiple substrates including Chk2, and it recruits ATR to form
part of an integrated repair circuit. Human ATM is 3056 amino acids (aa) in
length. It contains one FAT (focal adhesion targeting) domain (aa 1960-2566), a
PI-3/PI-4 kinase catalytic domain (aa 2712-2962) and a second C-terminal FAT
domain (aa 3024-3056). There are at least six Ser and four Thr utilized
phosphorylation sites, and one critical acetylation activation site at Lys3016.
Phosphorylation of Ser1981 is important for substrate recognition by ATM. There
are at least four potential splice variants. One shows a Trp substitution for
aa 536-3056, a second contains an eight aa substitution for aa 2506-3056, a
third possesses a five aa substitution for aa 1637-3056, while a fourth
contains a premature truncation after Lys2756. Over aa 1974-1987, human ATM
shares 79% aa identity with mouse ATM.
Entrez Gene IDs:
472 (Human); 11920 (Mouse)
AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutatedATD; ATC; ATDC; ATE; DKFZp781A0353; EC 220.127.116.11; MGC74674; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; TPLL
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