Human Phospho-Chk1 (S345) Antibody

Catalog # Availability Size / Price Qty
AF2475
AF2475-SP
Detection of Human Phospho-Chk1 (S345) by Western Blot.
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Human Phospho-Chk1 (S345) Antibody Summary

Species Reactivity
Human
Specificity
Detects human, mouse, and rat Chk1 when phosphorylated at S345. This antibody does not recognize Chk1 when unphosphorylated at S345.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human Chk1 S345 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-Chk1 (S345) by Western Blot. View Larger

Detection of Human Phospho-Chk1 (S345) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line exposed to 50 J/m2 UV-C with a 2 hour recovery. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-Chk1 (S345) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2475) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk1 (S345) was detected at approximately 55-60 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chk1

The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic or replicative stress, Chk1 is phosphorylated by the ATM or ATM-related kinase (ATR) at S345. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.

Long Name
Checkpoint Kinase 1
Entrez Gene IDs
1111 (Human); 12649 (Mouse); 140583 (Rat)
Alternate Names
Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); Chk1; CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 2.7.11.1

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