Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 849159
View all eIF2 alpha Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human eIF2 alpha S52 site
Accession # P05198

Specificity

Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human Phospho-eIF2 alpha (S52) Antibody

Detection of Human Phospho-eIF2a (S52) antibody by Western Blot.

Detection of Human Phospho-eIF2 alpha (S52) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes or 20 mJ/cm2 ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-eIF2a (S52) Monoclonal Antibody (Catalog # MAB39971) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-eIF2a (S52) at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Phospho-eIF2 alpha (S52) by Western Blot

Detection of Phospho-eIF2 alpha (S52) by Western Blot

Analysis of signal transduction during CHIKV infection in the presence of 50 nM silvestrol. (A) HEK 293T cells were seeded in six-well plates and were infected with CHIKV using an MOI of 1, and 50 nM silvestrol was added where indicated. Cells were treated with IFN alpha for 30 min before harvest if indicated, and Western blot analysis of cell lysates was performed. (−) Untreated cells; (+) treated cells. In lanes 3–6, cells were infected with CHIKV. The CHIKV E2 protein, STAT1, eIF2 alpha, and their phosphorylated proteins were detected with specific antibodies and secondary HRP-coupled antibodies, and the ECL detection system (Amersham, Freiburg). Equal loading of each blot was controlled by detection of beta -actin; and, (B) Uninfected HEK293T cells were treated with IFN alpha for 30 min before harvest and either treated with silvestrol for 16 h or left untreated. Western blot analysis of cell lysates was performed and p-STAT1 and STAT1 were detected. STAT1 served as a loading control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30380742), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Phospho-eIF2 alpha (S52) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA) or ultraviolet light (UV)

Reviewed Applications

Read 1 review rated 5 using MAB39971 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: eIF2 alpha

Eukaryotic translation initiation factor 2 alpha subunit (eIF2 alpha ) is a subunit of the eIF2 protein, an important regulator of translation initiation. Phosphorylation of eIF2 alpha on Ser 52 increases the affinity of eIF2 alpha for eIF2B, a guanine nucleotide exchange factor needed for the recycling of eIF2-GDP to eIF2-GTP. Reduction of eIF2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all eIF2 alpha kinases.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Entrez Gene IDs

1965 (Human); 13665 (Mouse); 54318 (Rat)

Gene Symbol

EIF2S1

UniProt

P05198

Additional eIF2 alpha Products

Product Documents for Human Phospho-eIF2 alpha (S52) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-eIF2 alpha (S52) Antibody

For research use only

Related Research Areas

Translation Regulation

Citations for Human Phospho-eIF2 alpha (S52) Antibody

Customer Reviews for Human Phospho-eIF2 alpha (S52) Antibody (1)

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  • Human Phospho-eIF2 alpha (S52) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Western blot
    Species: Human
    Verified Customer | Posted 05/11/2018
    works well
    Human Phospho-eIF2 alpha (S52) Antibody MAB39971

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