Human Phospho-eIF2 alpha (S52) Antibody

Catalog # Availability Size / Price Qty
MAB39971
MAB39971-SP
Detection of Human Phospho-eIF2 alpha  (S52) by Western Blot.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human Phospho-eIF2 alpha (S52) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots.
Source
Monoclonal Rat IgG2B Clone # 849159
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human eIF2 alpha S52 site
Accession # P05198
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-eIF2a (S52) antibody by Western Blot. View Larger

Detection of Human Phospho-eIF2 alpha (S52) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes or 20 mJ/cm2 ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-eIF2a (S52) Monoclonal Antibody (Catalog # MAB39971) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-eIF2a (S52) at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eIF2 alpha

Eukaryotic translation initiation factor 2 alpha subunit (eIF2 alpha ) is a subunit of the eIF2 protein, an important regulator of translation initiation. Phosphorylation of eIF2 alpha on Ser 52 increases the affinity of eIF2 alpha for eIF2B, a guanine nucleotide exchange factor needed for the recycling of eIF2-GDP to eIF2-GTP. Reduction of eIF2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all eIF2 alpha kinases.

Long Name
Eukaryotic Translation Initiation Factor 2 Alpha
Entrez Gene IDs
1965 (Human); 13665 (Mouse); 54318 (Rat)
Alternate Names
eIF2 alpha; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; EIF2S1

Product Datasheets

Citation for Human Phospho-eIF2 alpha (S52) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Silvestrol Inhibits Chikungunya Virus Replication
    Authors: L Henss, T Scholz, A Grünweller, BS Schnierle
    Viruses, 2018;10(11):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Phospho-eIF2 alpha (S52) Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Phospho-eIF2 alpha (S52) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Phospho-eIF2 alpha (S52) Antibody
By Anonymous on 05/11/2018
Application: Western Blot Sample Tested: Western blot Species: Human

works well