Human Phospho-Progesterone R/NR3C3 (S294) Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Phospho-Progesterone R/NR3C3 (S294) in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human Progesterone RB S294 site
    Accession # P06401
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-Progesterone RB (S294) and Phospho-Progesterone RA (S130) by Western Blot.

Western blot shows lysates of T47D human breast cancer cell line untreated (-) or treated (+) with 100 nM synthetic progestin promegestone (R5020) for 60 minutes. PVDF Membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Progesterone R/NR3C3 (S294) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5955) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-Progesterone RB (S294) at approximately XXX kDa and Phospho-Progesterone RA (S130) at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Progesterone R/NR3C3

The progesterone Receptor (PR; NR3C3) is a member of the steroid receptor superfamily which binds progesterone. The progesterone receptor is expressed in uterus, ovary, vagina, fallopian tubes and breast. Progesterone receptors play a central role in diverse reproductive events associated with the establishment and maintenance of pregnancy, alveolar development in the breast, and sexual behavior. Two isoforms of the progesterone receptor have been identified, progesterone receptor-A (PR-A) and progesterone receptor-B (PR-B), 94 and 114 kDa in size, respectively.

The B progesterone receptor (PR-B), or long form, is 933 amino acids (aa) in length. Progesterone receptor-A (PR-A) utilizes a different start site that shortens the N-terminus by 164 amino acids. The N-terminus in both progesterone receptor isoforms is rich in serine that is phosphorylated in response to hormone binding. In the absence of progesterone, few receptor molecules are phosphorylated at Serine 190 (S190). Progesterone increases this number two-fold, providing evidence for hormone stimulation. It has been shown that the two progesterone receptor isoforms can form homodimers as well as heterodimers.

  • Long Name:
    Progesterone Receptor
  • Entrez Gene IDs:
    5241 (Human)
  • Alternate Names:
    NR3C3; NR3C3PRNuclear receptor subfamily 3 group C member 3; PGR; Progesterone R; progesterone receptor; ProgesteroneR
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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