Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Progesterone R/NR3C3 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human Progesterone RB S294 site
Accession # P06401

Specificity

Detects human Phospho-Progesterone R/NR3C3 (S294) in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-Progesterone R/NR3C3 (S294) Antibody

Detection of Human Phospho-Progesterone RB (S294) and Phospho-Progesterone RA (S130) antibody by Western Blot.

Detection of Human Phospho-Progesterone RB (S294) and Phospho-Progesterone RA (S130) by Western Blot.

Western blot shows lysates of T47D human breast cancer cell line untreated (-) or treated (+) with 100 nM synthetic progestin promegestone (R5020) for 60 minutes. PVDF Membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Progesterone R/NR3C3 (S294) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5955) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-Progesterone RB (S294) at approximately 118 kDa and Phospho-Progesterone RA (S130) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-Progesterone R/NR3C3 (S294) Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: T47D human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Progesterone R/NR3C3

Progesterone Receptor B (PR-B) is a 118 kDa member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in female reproductive tissues as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR‑B is 933 amino acids (aa) in length. It contains an N-terminal regulatory region (aa 1‑566), a DNA binding domain (aa 567‑639), and a steroid‑binding region (aa 681‑933). Ligand binding induces a key phosphorylation event at Ser294 catalyzed by ERK1/2, regulating cell growth, cell adhesion, and gene transcription. An alternate start site at Met165 generates 90 kDa PR-A, an isoform particularly important in the ovary and uterus that insures fertility.

Long Name

Progesterone Receptor

Alternate Names

NR3C3, PGR, ProgesteroneR

Entrez Gene IDs

5241 (Human)

Gene Symbol

PGR

UniProt

P06401

Additional Progesterone R/NR3C3 Products

Product Documents for Human Phospho-Progesterone R/NR3C3 (S294) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-Progesterone R/NR3C3 (S294) Antibody

For research use only

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